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Broadly expressed repressors integrate patterning across orthogonal axes in embryos

Koromila, Theodora and Stathopoulos, Angelike (2017) Broadly expressed repressors integrate patterning across orthogonal axes in embryos. Proceedings of the National Academy of Sciences of the United States of America, 114 (31). pp. 8295-8300. ISSN 0027-8424. PMCID PMC5547611. https://resolver.caltech.edu/CaltechAUTHORS:20170718-100508696

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Abstract

The role of spatially localized repressors in supporting embryonic patterning is well appreciated, but, alternatively, the role ubiquitously expressed repressors play in this process is not well understood. We investigated the function of two broadly expressed repressors, Runt (Run) and Suppressor of Hairless [Su(H)], in patterning the Drosophila embryo. Previous studies have shown that Run and Su(H) regulate gene expression along anterior-posterior (AP) or dorsal-ventral (DV) axes, respectively, by spatially limiting activator action, but here we characterize a different role. Our data show that broadly expressed repressors silence particular enhancers within cis-regulatory systems, blocking their expression throughout the embryo fully but transiently, and, in this manner, regulate spatiotemporal outputs along both axes. Our results suggest that Run and Su(H) regulate the temporal action of enhancers and are not dedicated regulators of one axis but, instead, act coordinately to pattern both axes, AP and DV.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1073/pnas.1703001114DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5547611/PubMed CentralArticle
http://www.pnas.org/lookup/suppl/doi:10.1073/pnas.1703001114/-/DCSupplementalPublisherSupporting Information
ORCID:
AuthorORCID
Stathopoulos, Angelike0000-0001-6597-2036
Additional Information:© 2017 National Academy of Sciences. Edited by Norbert Perrimon, Harvard Medical School, Boston, MA, and approved June 23, 2017 (received for review February 21, 2017). Published online before print July 18, 2017. We thank Peter Gergen, Mike Levine, James Posakony, and Stephen Small for providing plasmids and fly stocks and the A.S. laboratory, Lijia Ma, Andres Collazo, and Henry Amrhein for experimental support and helpful discussions. This study was supported by NIH Grants R35GM118146 and GM077668 (to A.S.). Author contributions: T.K. and A.S. designed research; T.K. performed research; T.K. and A.S. analyzed data; and T.K. and A.S. wrote the paper. The authors declare no conflict of interest. This article is a PNAS Direct Submission. This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1703001114/-/DCSupplemental.
Funders:
Funding AgencyGrant Number
NIHR35GM118146
NIHGM077668
Subject Keywords:embryonic patterning; transcriptional repressor; enhancers; Su(H); Runt
Issue or Number:31
PubMed Central ID:PMC5547611
Record Number:CaltechAUTHORS:20170718-100508696
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170718-100508696
Official Citation:Theodora Koromila and Angelike Stathopoulos Broadly expressed repressors integrate patterning across orthogonal axes in embryos PNAS 2017 114 (31) 8295-8300; published ahead of print July 18, 2017, doi:10.1073/pnas.1703001114
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:79148
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:18 Jul 2017 18:08
Last Modified:03 Oct 2019 18:16

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