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Response to Comments on “The [4Fe4S] cluster of human DNA primase functions as a redox switch using DNA charge transport”

O'Brien, Elizabeth and Holt, Marilyn E. and Thompson, Matthew K. and Salay, Lauren E. and Ehlinger, Aaron C. and Chazin, Walter J. and Barton, Jacqueline K. (2017) Response to Comments on “The [4Fe4S] cluster of human DNA primase functions as a redox switch using DNA charge transport”. Science, 357 (6348). Art. No. 2762. ISSN 0036-8075. PMCID PMC5935490. doi:10.1126/science.aan2762. https://resolver.caltech.edu/CaltechAUTHORS:20170720-130038340

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Abstract

Baranovskiy et al. and Pellegrini argue that, based on structural data, the path for charge transfer through the [4Fe4S] domain of primase is not feasible. Our manuscript presents electrochemical data directly showing charge transport through DNA to the [4Fe4S] cluster of a primase p58C construct and a reversible switch in the DNA-bound signal with oxidation/reduction, which is inhibited by mutation of three tyrosine residues. Although the dispositions of tyrosines differ in different constructs, all are within range for microsecond electron transfer.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/ 10.1126/science.aan2762DOIArticle
http://science.sciencemag.org/content/357/6348/eaan2762PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5935490PubMed CentralArticle
ORCID:
AuthorORCID
Holt, Marilyn E.0000-0002-3164-869X
Chazin, Walter J.0000-0002-2180-0790
Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 2017 American Association for the Advancement of Science. 10 April 2017; accepted 13 June 2017.
Issue or Number:6348
PubMed Central ID:PMC5935490
DOI:10.1126/science.aan2762
Record Number:CaltechAUTHORS:20170720-130038340
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170720-130038340
Official Citation:Response to Comments on “The [4Fe4S] cluster of human DNA primase functions as a redox switch using DNA charge transport” BY ELIZABETH O’BRIEN, MARILYN E. HOLT, MATTHEW K. THOMPSON, LAUREN E. SALAY, AARON C. EHLINGER, WALTER J. CHAZIN, JACQUELINE K. BARTON SCIENCE 21 JUL 2017
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:79253
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:20 Jul 2017 20:21
Last Modified:15 Nov 2021 17:46

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