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Mutations in a C. elegans G_qα Gene Disrupt Movement, Egg Laying, and Viability

Brundage, Lorna and Avery, Leon and Katz, Arieh and Kim, Ung-Jin and Mendel, Jane E. and Sternberg, Paul W. and Simon, Melvin I. (1996) Mutations in a C. elegans G_qα Gene Disrupt Movement, Egg Laying, and Viability. Neuron, 16 (5). pp. 999-1009. ISSN 0896-6273. PMCID PMC4444781. https://resolver.caltech.edu/CaltechAUTHORS:20170721-070445218

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Abstract

We find that C. elegans egl-30 encodes a heterotrimeric G protein α subunit more than 80% identical to mammalian G_qα family proteins, and which can function as a G_qα subunit in COS-7 cells. We have identified new egl-30 alleles in a selection for genes involved in the C. elegans acetylcholine response. Two egl-30 alleles specify premature termination of G_qα and are essentially lethal in homozygotes. Animals homozygous for six other egl-30 alleles are viable and fertile, but exhibit delayed egg laying and leave flattened tracks. Overexpression of the wild-type egl-30 gene produces the opposite behavior. Analysis of these mutants suggest that their phenotypes reflect defects in the muscle or neuromuscular junction.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/S0896-6273(00)80123-3DOIArticle
http://www.sciencedirect.com/science/article/pii/S0896627300801233PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4444781/PubMed CentralArticle
ORCID:
AuthorORCID
Sternberg, Paul W.0000-0002-7699-0173
Additional Information:© 1996 Cell Press. Received 6 November 1995, Revised 22 March 1996. We thank Harry Duttweiler, who selected and partially backcrossed the eat-11 suppressors, and Steve Marsh and Rick Colayco (Caltech DNA sequencing core facility) for assistance in determining the egl-30 lesions. The C. elegans Genetic Stock Center provided some of the strains described here. This work was funded by a grant from the Human Frontiers in Science Organization to M. I. S and P. W. S., research grant HL46154 from the United States Public Health Service to L. A., and National Research Service Award F32NS09541–03 to L. B. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 USC Section 1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
Human Frontier Science ProgramUNSPECIFIED
NIHHL46154
NIH Postodoctoral FellowshipF32NS09541–03
Issue or Number:5
PubMed Central ID:PMC4444781
Record Number:CaltechAUTHORS:20170721-070445218
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170721-070445218
Official Citation:Lorna Brundage, Leon Avery, Arieh Katz, Ung-Jin Kim, Jane E Mendel, Paul W Sternberg, Melvin I Simon, Mutations in a C. elegans Gqα Gene Disrupt Movement, Egg Laying, and Viability, Neuron, Volume 16, Issue 5, May 1996, Pages 999-1009, ISSN 0896-6273, https://doi.org/10.1016/S0896-6273(00)80123-3. (http://www.sciencedirect.com/science/article/pii/S0896627300801233)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:79263
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:21 Jul 2017 17:49
Last Modified:03 Oct 2019 18:18

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