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Photon-induced near-field electron microscopy (PINEM) of eukaryotic cells

Kaplan, Mohammed and Yoo, Byung-Kuk and Tang, Jau and Karam, Tony E. and Liao, Bolin and Majumdar, Devdoot and Baltimore, David and Jensen, Grant J. and Zewail, Ahmed H. (2017) Photon-induced near-field electron microscopy (PINEM) of eukaryotic cells. Angewandte Chemie International Edition, 56 (38). pp. 11498-11501. ISSN 1433-7851. https://resolver.caltech.edu/CaltechAUTHORS:20170724-124156529

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Abstract

Photon-induced near-field electron microscopy (PINEM) is a technique to produce and then image evanescent electromagnetic fields on the surfaces of nanostructures. Most previous applications of PINEM have imaged surface plasmon-polariton waves on conducting nanomaterials. Here, the application of PINEM on whole human cancer cells and membrane vesicles isolated from them is reported. We show that photons induce time-, orientation-, and polarization-dependent evanescent fields on the surfaces of A431 cancer cells and isolated membrane vesicles. Furthermore, the addition of a ligand to the major surface receptor on these cells and vesicles (Epidermal Growth Factor Receptor, EGFR) reduces the intensity of these fields in both preparations. In the absence of plasmon waves in biological samples, we propose these evanescent fields reflect the changes of EGFR kinase domain polarization upon ligand binding.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1002/anie.201706120DOIArticle
http://onlinelibrary.wiley.com/doi/10.1002/anie.201706120/abstractPublisherArticle
ORCID:
AuthorORCID
Kaplan, Mohammed0000-0002-0759-0459
Yoo, Byung-Kuk0000-0002-2610-6685
Tang, Jau0000-0003-2078-1513
Karam, Tony E.0000-0002-1244-5141
Liao, Bolin0000-0002-0898-0803
Baltimore, David0000-0001-8723-8190
Jensen, Grant J.0000-0003-1556-4864
Additional Information:© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. Manuscript received: June 15, 2017; Accepted manuscript online: July 23, 2017; Version of record online: August 10, 2017. We thank Spencer Baskin and Mohammed Hassan for helpful discussions. This work was supported by the National Science Foundation Grant DMR-0964886 and the Air Force Office of Scientific Research Grant FA9550-11-1-0055 for research conducted in The Gordon and Betty Moore Center for Physical Biology at the California Institute of Technology. The authors declare no conflict of interest.
Funders:
Funding AgencyGrant Number
NSFDMR-0964886
Air Force Office of Scientific Research (AFOSR)FA9550-11-1-0055
Gordon and Betty Moore FoundationUNSPECIFIED
Subject Keywords:electron microscopy; electron–photon coupling; eukaryotic cells; femtobiology; PINEM
Issue or Number:38
Record Number:CaltechAUTHORS:20170724-124156529
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170724-124156529
Official Citation:M. Kaplan, B.-K. Yoo, J. Tang, T. E. Karam, B. Liao, D. Majumdar, D. Baltimore, G. J. Jensen, A. H. Zewail, Angew. Chem. Int. Ed. 2017, 56, 11498
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:79304
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:24 Jul 2017 19:56
Last Modified:03 Oct 2019 18:18

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