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Cell-free extract based optimization of biomolecular circuits with droplet microfluidics

Hori, Yutaka and Kantak, Chaitanya and Murray, Richard M. and Abate, Adam R. (2017) Cell-free extract based optimization of biomolecular circuits with droplet microfluidics. Lab on a Chip, 17 (18). pp. 3037-3042. ISSN 1473-0197. https://resolver.caltech.edu/CaltechAUTHORS:20170807-111733897

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Abstract

Engineering an efficient biomolecular circuit often requires time-consuming iterations of optimization. Cell-free protein expression systems allow rapid testing of biocircuits in vitro, speeding the design–build–test cycle of synthetic biology. In this paper, we combine this with droplet microfluidics to densely scan a transcription–translation biocircuit space. Our system assays millions of parameter combinations per hour, providing a detailed map of function. The ability to comprehensively map biocircuit parameter spaces allows accurate modeling to predict circuit function and identify optimal circuits and conditions.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1039/C7LC00552KDOIArticle
http://pubs.rsc.org/en/content/articlehtml/2017/lc/c7lc00552kPublisherArticle
ORCID:
AuthorORCID
Hori, Yutaka0000-0002-3253-4985
Kantak, Chaitanya0000-0003-1620-7084
Murray, Richard M.0000-0002-5785-7481
Abate, Adam R.0000-0001-9614-4831
Additional Information:© 2017 The Royal Society of Chemistry. Received 23rd May 2017, Accepted 27th July 2017. First published on 3rd August 2017. The authors would like to thank Shaobin Guo at Caltech for providing the plasmids of the IFFL circuit used in this study. This work was supported by the Defense Advanced Research Projects Agency (DARPA) under Living Foundries program (HR0011-12- C-0065). Y. Hori was supported by JSPS Fellowship for Research Abroad.
Funders:
Funding AgencyGrant Number
Defense Advanced Research Projects Agency (DARPA)HR0011-12- C-0065
Japan Society for the Promotion of Science (JSPS)UNSPECIFIED
Issue or Number:18
Record Number:CaltechAUTHORS:20170807-111733897
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170807-111733897
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:79845
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:07 Aug 2017 18:36
Last Modified:03 Oct 2019 18:24

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