Sequence of the R1-11 P450 BM3 heme domain variant that formed the parent for the neutral evolution, and a listing of the mutations occurring in the 34 neutrally evolved variants that were assayed for activity.  The amino acid mutations are numbered according to the standard P450 numbering scheme in which the number one is assigned to the threonine that occurs after the N-terminal methionine.

R1-11 parent DNA sequence : ATGACTATTAAAGAAATGCCTCAGCCAAAAGCGTTTGGAGAGCTTAAAAATTTACCGTTATTAAACACAGATAAACCGGTTCAAGCCTTGATGAAATTTGCGGATGAATTAGGAGAAATCTTTAAATTCGAGGTGCCAGGTCGTGTAACGCGCTACTTATCAAGTCAGCGTCTAGTTAAAGAAGCATGCGATGAATCTCGCTTTGATAAAAACTTAAGTCAAGCGCTTAAATTTGTACGGGATTTTGCAGGAGACGGGTTGGCCACAAGCTGGACGCATGAAAAAAATTGGAAAAAAGCGCGTAATATCCTACTTCCAAGCTTAAGTCAGCAGGCAATGAAAGGCTATCATGCGATGATGGTCGATATCGCCGTGCAGCTTGTTCAAAAGTGGGAGCGACTAAATTCAGATGAGCATATTGAAGTATCGGAAGACGTGACACGTTTAACGCTTGATACGATTGGTCTTTGCGGCTTTAACTATCGCTTTAACAGCTTTTACCGAGATCAGCCTCATCCATTTATTACAAGTATGGTCCGTGCACTGGATGACGCAGTGAACAAGCTGCAGCGAGTAAATCCAGACGACCCAGCTTATGAAGAGAATGAGCGCCTGTTTCAAGAAGATATCAAGGTGATGAACGACCTAGTAGATAAAATTATTGCAGATCGCAAAGCAAGCGGTGAGCAAAGTGATGATTTATTAACGCGCATGCTACACGGAAAAGATCCAGAAACGGGTGAGCCGCTTGATGACGAGAACATTCGCTATCAAATTATTACATTCTTGATTGCGGGACACGAAACAACAAGCGGTCTTCTAACATTTGCGCTGTATTTCTTAGTGAAAAATCCACATGTATTACAAGAAGCAGCAGAAGAAGCAGCGCGGGTTCTAGTAGATCCTGTTCCAAGCTACAAACAAGTCAAACAGCTCAAATATGTCGGCATGGTCTTAAACGAAGCGCTGCGCTTATGGCCAACAGCTCCTGCGTTTTCCCTATATGCAAAAGAAGATACGGTGCTTGGAGGAGAATATCCTTTAGAAAAAGGCGACGAACTAATGGTTCTGATTCCTCAGCTTCACCGTGATAAAACAATTTGGGGAGACGATGTGGAAGAGTTCCGTCCAGAGCGTTTTGAAAATCCAGGTGCGATTCCGCAGCATGCGTTTAAACCGTTTGGAAACGGACAGCGTGCGTGTATCGGTCAGCAGTTCGCCCTTCATGAAGCAACGCTGGTACTTGGTATGATGCTAAAACACTTTGACTTTGAAGATCATACAAACTACGAGCTGGATATTGAAGAAACTTTAACGTTAAAACCTGAAGGCTTTGTGATAAAAGCAAAATCGAAAAAAATTCCGCTTGGCGGTATTCCTTCACCTAGCACTCATCATCATCATCATCATTAA

P1 : A67G (T22A), T72C (SYNONYMOUS), G152A (R50H), T406C (S135P), G438A (SYNONYMOUS), A723C (SYNONYMOUS), A882G (SYNONYMOUS), A959G (N319S), G1080A (SYNONYMOUS), T1152C (SYNONYMOUS), A1274T (E424V)
P3 : T36C (SYNONYMOUS), A48G (SYNONYMOUS), T97C (F32L), C129T (SYNONYMOUS), G152T (R50L), A183G (SYNONYMOUS), C369T (SYNONYMOUS), A431G (E143G), G438A (SYNONYMOUS), A504T (SYNONYMOUS), A752G (D250G), G1021A (V340M), A1339T (I446L), T1380C (SYNONYMOUS)
P4 : A28G (K9E), T219C (SYNONYMOUS), T233A (F77Y), A282G (SYNONYMOUS), A367G (I122V), A666C (SYNONYMOUS), C746T (P248L), G891A (SYNONYMOUS), A1059G (SYNONYMOUS), C1069T (SYNONYMOUS), T1411C (NOSTOP), A1413T (NOSTOP)
P6 : C363T (SYNONYMOUS), C397A (SYNONYMOUS), A591G (SYNONYMOUS), T663A (SYNONYMOUS), T693G (S230R), T696C (SYNONYMOUS), T784A (F261I), A879C (E292D), T942C (SYNONYMOUS), G1045A (E348K), A1234G (T411A), A1260G (SYNONYMOUS), A1329G (SYNONYMOUS), T1376G (I458S), A1383T (SYNONYMOUS)
P8 : T45C (SYNONYMOUS), T61C (SYNONYMOUS), T144C (SYNONYMOUS), G152A (R50H), A159G (SYNONYMOUS), A183T (E60D), A285G (SYNONYMOUS), T406C (S135P), G438A (SYNONYMOUS), A755T (D251V), T1017A (D338E), T1152A (SYNONYMOUS), delCAT1408: (FRAMESHIFT)
P9 : T279C (SYNONYMOUS), C630T (SYNONYMOUS), G636A (SYNONYMOUS), A657G (SYNONYMOUS), T996C (SYNONYMOUS), A1049G (K349R), A1242C (SYNONYMOUS), T1278C (SYNONYMOUS)
P10 : T61C (SYNONYMOUS), T144C (SYNONYMOUS), G152A (R50H), A183T (E60D), A285G (SYNONYMOUS), T406C (S135P), G438A (SYNONYMOUS), T669C (SYNONYMOUS), A798G (SYNONYMOUS), A867T (Q288H), A1144G (N381D)
P11 : G152A (R50H), A395C (E131A), T684G (SYNONYMOUS), T903C (SYNONYMOUS), A932G (Q310R), A1032C (SYNONYMOUS), A1107T (SYNONYMOUS), A1400G (H466R), A1409T (H469L)
P13 : A7G (I2V), A147T (SYNONYMOUS), G276C (SYNONYMOUS), A285G (SYNONYMOUS), T406C (S135P), G438A (SYNONYMOUS), A548G (D182G), A666C (SYNONYMOUS), T819C (SYNONYMOUS), A823T (T274S), T858C (SYNONYMOUS), T996C (SYNONYMOUS), A1049G (K349R), A1242C (SYNONYMOUS), T1278C (SYNONYMOUS), A1383T (SYNONYMOUS)
P15 : T444C (SYNONYMOUS), A651G (SYNONYMOUS), A1191T (SYNONYMOUS), C1405G (H468D)
P16 : T207A (D68E), A242G (D80G), T453A (SYNONYMOUS), A735G (SYNONYMOUS), T750C (SYNONYMOUS), T996C (SYNONYMOUS), A1242C (SYNONYMOUS), T1278C (SYNONYMOUS), T1411C (NOSTOP)
P17 : A282G (SYNONYMOUS), G373A (V124M), A399T (SYNONYMOUS), A604G (N201D), A810G (SYNONYMOUS), A1234G (T411A)
P18 : T72C (SYNONYMOUS), T141A (SYNONYMOUS), G152A (R50H), T279C (SYNONYMOUS), T684G (SYNONYMOUS), T903C (SYNONYMOUS), T996C (SYNONYMOUS), A1049G (K349R), A1242C (SYNONYMOUS), T1278C (SYNONYMOUS), A1383T (SYNONYMOUS)
P19 : T171G (SYNONYMOUS), A441G (SYNONYMOUS), A447G (SYNONYMOUS), A604G (N201D), A823T (T274S), A1230G (SYNONYMOUS), T1272C (SYNONYMOUS), T1386A (SYNONYMOUS)
P20 : G152A (R50H), A312G (SYNONYMOUS), T684G (SYNONYMOUS), A855G (SYNONYMOUS), A882G (SYNONYMOUS), T903C (SYNONYMOUS), T1209C (SYNONYMOUS), A1350G (SYNONYMOUS), T1392A (SYNONYMOUS)
P22 : T207A (D68E), A242G (D80G), T453A (SYNONYMOUS), A783G (SYNONYMOUS), A870G (SYNONYMOUS), T894A (SYNONYMOUS), T1115C (V371A), T1152C (SYNONYMOUS), A1285G (N428D), C1391A (T463N), A1413T (NOSTOP)
P23 : A69C (SYNONYMOUS), T105C (SYNONYMOUS), A148G (T49V), C149T (T49V*), A297G (SYNONYMOUS), C581T (P193L), A628G (I209V), A825T (SYNONYMOUS), A939G (SYNONYMOUS), T996C (SYNONYMOUS), C1235A (T411K), A1356G (SYNONYMOUS), A1413G (SYNONYMOUS)
P24 : A147T (SYNONYMOUS), G438A (SYNONYMOUS), A641C (N213T), T903C (SYNONYMOUS), A1242C (SYNONYMOUS), T1278C (SYNONYMOUS)
M1 : T496C (F165L), T1101A (SYNONYMOUS), T1299A (D432E), T1311C (SYNONYMOUS)
M2 : T98A (F32Y), C224T (A74V), C277T (H92Y), T462A (SYNONYMOUS), T792C (SYNONYMOUS), A921C (K306N), A1016G (D338G), A1252G (M417V)
M3 : A403G (N134G), A404G (N134G*), A657G (SYNONYMOUS), A861G (SYNONYMOUS), A975G (SYNONYMOUS), A1094G (K364R), A1131T (SYNONYMOUS), A1357G (K452E), T1386A (SYNONYMOUS)
M7 : C153T (SYNONYMOUS), A605C (N201T), A624C (E207D), A732G (SYNONYMOUS), A849G (SYNONYMOUS), A1037T (Y345F), A1046C (E348A), T1197G (SYNONYMOUS), A1394G (H464R)
M8 : A27G (SYNONYMOUS), A84G (SYNONYMOUS), A125T (K41I), A509T (Q169L), G574T (V191L), G759A (SYNONYMOUS), A763G (I254V), A885G (SYNONYMOUS), T942C (SYNONYMOUS), C992T (A330V), A1131T (SYNONYMOUS), delCATCAT1405: (FRAMESHIFT)
M11 : A876G (SYNONYMOUS), T994A (F331I), T996C (SYNONYMOUS), A1359G (SYNONYMOUS), C1366T (L455F), T1380A (SYNONYMOUS)
M12 : A147G (SYNONYMOUS), T356C (M118T), T507A (D168E), T618A (F205L), A1062T (SYNONYMOUS), delCAT1408: (FRAMESHIFT) 
M13 : G31T (A10S), T61C (SYNONYMOUS), T88C (SYNONYMOUS), A252G (SYNONYMOUS), A274T (T91S), A624G (SYNONYMOUS), A668G (D222G), A1014G (SYNONYMOUS), A1029C (SYNONYMOUS), G1065A (M354I), A1143C (E380D), C1391A (T463N), T1411C (NOSTOP), A1412G (SYNONYMOUS)
M14 : T98C (F32S)
M15 : T8A (I2N), T72C (SYNONYMOUS), T141C (SYNONYMOUS), C149T (T49M), G223C (A74P), A563G (K187R), T753C (SYNONYMOUS), A823T (T274S), A1072G (I357V), T1318C (SYNONYMOUS)
M19 : T196A (S65T), A422T (E140V), A686T (E228V), T699C (SYNONYMOUS), C756T (SYNONYMOUS), T816C (SYNONYMOUS), C889T (R296W), T1068A (SYNONYMOUS), T1407A (H468Q)
M20 : A69T (SYNONYMOUS), T322C (SYNONYMOUS), A408G (SYNONYMOUS), T1042A (L347I), A1260G (SYNONYMOUS), A1322G (K440R), A1390G (T463A), delCAT1408: (FRAMESHIFT)
M21 : T97A (F32I), A206G (D68G), T233A (F77Y), A578G (N192S), T816C (SYNONYMOUS), A847C (K282Q), T1041C (SYNONYMOUS), G1370T (G456V), A1413G (SYNONYMOUS)
M22 : T6A (SYNONYMOUS), T109C (SYNONYMOUS), T246C (SYNONYMOUS), T381A (SYNONYMOUS), T444C (SYNONYMOUS), T597C (SYNONYMOUS), G757A (E252K), A882G (SYNONYMOUS), A958T (N319Y), T990C (SYNONYMOUS), A1322G (K440R), A1359G (SYNONYMOUS)
M23 : A312G (SYNONYMOUS), T406C (S135P), A560T (N186I), T741C (SYNONYMOUS), G888A (SYNONYMOUS), T1146A (N381K), T1374C (SYNONYMOUS)
M24 : G443A (R147H), A790G (I263V), T1264C (F421L), A1328T (E442V)