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Granulocytes as models for human protein marker identification following nicotine exposure

Mulcahy, Matthew J. and Lester, Henry A. (2017) Granulocytes as models for human protein marker identification following nicotine exposure. Journal of Neurochemistry, 142 (S2). pp. 151-161. ISSN 0022-3042. PMCID PMC6057152. doi:10.1111/jnc.14010.

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Nicotinic acetylcholine receptors (nAChRs) are pentameric cation channels expressed in the mammalian CNS, in the peripheral nervous system, and in skeletal muscle. Neuronal-type nAChRs are also found in several non-neuronal cell types, including leukocytes. Granulocytes are a subtype of leukocytes that include basophils, eosinophils, and neutrophils. Granulocytes, also known as polymorphonuclear leukocytes, are characterized by their ability to produce, store, and release compounds from intracellular granules. Granulocytes are the most abundant type of leukocyte circulating in the peripheral blood. Granulocyte abundance, nAChR expression, and nAChR upregulation following chronic nicotine administration makes granulocytes interesting models for identifying protein markers of nicotine exposure. Nicotinic receptor subunits and several non-nAChR proteins have been identified as protein markers of granulocyte nicotine exposure. We review methods to isolate granulocytes from human tissue, summarize present data about the expression of nAChRs in the three granulocyte cell types (basophils, eosinophils, and neutrophils), describe current knowledge of the effects of nicotine exposure on human granulocyte protein expression, and highlight areas of interest for future investigation.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Mulcahy, Matthew J.0000-0001-5588-8762
Lester, Henry A.0000-0002-5470-5255
Additional Information:© 2017 International Society for Neurochemistry. Issue online: 8 August 2017; Version of record online: 8 August 2017; Manuscript Accepted: 1 March 2017; Manuscript Revised: 28 February 2017; Manuscript Received: 16 November 2016. This work was funded by National Institutes of Health (DA036061). The authors have no conflicts of interest to declare.
Funding AgencyGrant Number
Subject Keywords:granulocytes; nAChRs; nicotine
Issue or Number:S2
PubMed Central ID:PMC6057152
Record Number:CaltechAUTHORS:20170809-134755993
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Official Citation:Mulcahy, M. J. and Lester, H. A. (2017), Granulocytes as models for human protein marker identification following nicotine exposure. J. Neurochem., 142: 151–161. doi:10.1111/jnc.14010
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:80024
Deposited By: Tony Diaz
Deposited On:09 Aug 2017 21:06
Last Modified:04 Apr 2022 23:17

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