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Imaging small animal whole-body dynamics by single-impulse panoramic photoacoustic computed tomography

Li, Lei and Zhu, Liren and Ma, Cheng and Lin, Li and Yao, Junjie and Wang, Lidai and Maslov, Konstantin and Zhang, Ruiying and Chen, Wanyi and Shi, Junhui and Wang, Lihong V. (2017) Imaging small animal whole-body dynamics by single-impulse panoramic photoacoustic computed tomography. In: Photons Plus Ultrasound: Imaging and Sensing 2017. Proceedings of SPIE. No.10064. Society of Photo-Optical Instrumentation Engineers (SPIE) , Bellingham, WA, Art. No. 100640M. ISBN 978-1-5106-0569-5. http://resolver.caltech.edu/CaltechAUTHORS:20170810-081618851

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Abstract

Small animal whole-body imaging, providing physiological, pathological, and phenotypical insights into biological processes, is indispensable in preclinical research. With high spatiotemporal resolution and functional contrast, small animal imaging can visualize biological dynamics in vivo at whole-body scale, which can advance both fundamental biology and translational medicine. However, current non-optical imaging techniques lack either spatiotemporal resolution or functional contrasts, and pure optical imaging suffers from either shallow penetration (up to ~1 mm) or a poor resolution-to-depth ratio (~1/3). Here, we present a standalone system, termed single-impulse panoramic photoacoustic computed tomography (SIP-PACT), which overcomes all the above limitations. Our technology, with unprecedented performance, is envisioned to complement existing modalities for imaging entire small animals. As an optical imaging modality, SIP-PACT captures the high molecular contrast of endogenous substances such as hemoglobin, melanin, and lipid, as well as exogenous biomarkers, at the whole animal scale with full-view fidelity. Unlike other optical imaging methods, SIP-PACT sees through ~5 cm of tissue in vivo, and acquires cross-sectional images with an in-plane resolution of ~100 μm. Such capabilities allow us to image, for the first time, mouse wholebody dynamics in real time with clear sub-organ anatomical and functional details and without motion artifacts. SIPPACT can capture transients of whole-body oxygen saturation and pulse wave propagation in vivo without labeling. In sum, we expect widespread applications of SIP-PACT as a whole-body imaging tool for small animals in fundamental biology, pharmacology, pathology, oncology, and other areas.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1117/12.2251593DOIArticle
http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=2614006PublisherArticle
ORCID:
AuthorORCID
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2017 Society of Photo-Optical Instrumentation Engineers (SPIE). We thank Yun He, Chiye Li, Yang Li, and Jun Xia for technical support, and James Ballard for close reading of the manuscript. This work was sponsored by the US National Institutes of Health grants DP1 EB016986 (NIH Director’s Pioneer Award), R01 CA186567 (NIH Director’s Transformative Research Award), U01 NS090579 (BRAIN Initiative), R01 EB016963, and S10 RR026922.
Funders:
Funding AgencyGrant Number
NIHDP1 EB016986
NIHR01 CA186567
NIHU01 NS090579
NIHR01 EB016963
NIHS10 RR026922
Subject Keywords:Single-impulse, whole-body imaging, small-animal imaging, photoacoustic computed tomography
Record Number:CaltechAUTHORS:20170810-081618851
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20170810-081618851
Official Citation:Lei Li ; Liren Zhu ; Cheng Ma ; Li Lin ; Junjie Yao ; Lidai Wang ; Konstantin Maslov ; Ruiying Zhang ; Wanyi Chen ; Junhui Shi ; Lihong V. Wang; Imaging small animal whole-body dynamics by single-impulse panoramic photoacoustic computed tomography. Proc. SPIE 10064, Photons Plus Ultrasound: Imaging and Sensing 2017, 100640M (March 23, 2017); doi:10.1117/12.2251593.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:80056
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:10 Aug 2017 16:41
Last Modified:10 Aug 2017 16:41

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