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Real-time photoacoustic flow cytography and photothermolysis of single circulating melanoma cells in vivo

He, Yun and Wang, Lidai and Shi, Junhui and Yao, Junjie and Li, Lei and Zhang, Ruiying and Huang, Chih-Hsien and Zou, Jun and Wang, Lihong V. (2017) Real-time photoacoustic flow cytography and photothermolysis of single circulating melanoma cells in vivo. In: Photons Plus Ultrasound: Imaging and Sensing 2017. Proceedings of SPIE. No.10064. Society of Photo-Optical Instrumentation Engineers (SPIE) , Bellingham, WA, Art. No. 100641E. ISBN 978-1-5106-0569-5. http://resolver.caltech.edu/CaltechAUTHORS:20170810-083659632

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Abstract

Metastasis is responsible for as many as 90% of cancer-related deaths, and the deadliest skin cancer, melanoma, has a high propensity for metastasis. Since hematogenous spread of circulating tumor cells (CTCs) is cancer’s main route of metastasis, detecting and destroying CTCs can impede metastasis and improve patients’ prognoses. Extensive studies employing exogenous agents to detect tumor-specific biomarkers and guide therapeutics to CTCs have achieved promising results, but biosafety remains a critical concern. Taking another approach, physical detection and destruction of CTCs is a safer way to evaluate and reduce metastasis risks. Melanoma cells strongly express melanosomes, providing a striking absorption contrast with the blood background in the red to near-infrared spectrum. Exploiting this intrinsic optical absorption contrast of circulating melanoma cells, we coupled dual-wavelength photoacoustic flow cytography with a nanosecond-pulsed laser killing mechanism that specifically targets melanoma CTCs. We have successfully achieved in vivo label-free imaging of rare single CTCs and CTC clusters in mice. Further, the photoacoustic signal from a CTC immediately hardware-triggers a lethal pinpoint laser irradiation that lyses it on the spot in a thermally confined manner. Our technology can facilitate early inhibition of metastasis by clearing circulating tumor cells from vasculature.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1117/12.2255068DOIArticle
http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=2614009PublisherArticle
ORCID:
AuthorORCID
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2017 Society of Photo-Optical Instrumentation Engineers (SPIE).
Subject Keywords:Photoacoustic microscopy, flow cytography, circulating tumor cell, melanoma, laser therapy
Record Number:CaltechAUTHORS:20170810-083659632
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20170810-083659632
Official Citation:Yun He ; Lidai Wang ; Junhui Shi ; Junjie Yao ; Lei Li ; Ruiying Zhang ; Chih-Hsien Huang ; Jun Zou ; Lihong V. Wang; Real-time photoacoustic flow cytography and photothermolysis of single circulating melanoma cells in vivo. Proc. SPIE 10064, Photons Plus Ultrasound: Imaging and Sensing 2017, 100641E (March 23, 2017); doi:10.1117/12.2255068.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:80057
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:10 Aug 2017 16:36
Last Modified:10 Aug 2017 16:36

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