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Fourier Ptychographic Microscopy for Rapid, High-Resolution Imaging of Circulating Tumor Cells Enriched by Microfiltration

Williams, Anthony and Chung, Jaebum and Yang, Changhuei and Cote, Richard J. (2017) Fourier Ptychographic Microscopy for Rapid, High-Resolution Imaging of Circulating Tumor Cells Enriched by Microfiltration. In: Circulating Tumor Cells. Methods in Molecular Biology. No.1634. Humana Press , New York, NY, pp. 107-117. ISBN 978-1-4939-7143-5.

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Examining the hematogenous compartment for evidence of metastasis has increased significantly within the oncology research community in recent years, due to the development of technologies aimed at the enrichment of circulating tumor cells (CTCs), the subpopulation of primary tumor cells that gain access to the circulatory system and are responsible for colonization at distant sites. In contrast to other technologies, filtration-based CTC enrichment, which exploits differences in size between larger tumor cells and surrounding smaller, non-tumor blood cells, has the potential to improve CTC characterization through isolation of tumor cell populations with greater molecular heterogeneity. However, microscopic analysis of uneven filtration surfaces containing CTCs is laborious, time-consuming, and inconsistent, preventing widespread use of filtration-based enrichment technologies. Here, integrated with a microfiltration-based CTC and rare cell enrichment device we have previously described, we present a protocol for Fourier Ptychographic Microscopy (FPM), a method that, unlike many automated imaging platforms, produces high-speed, high-resolution images that can be digitally refocused, allowing users to observe objects of interest present on multiple focal planes within the same image frame. The development of a cost-effective and high-throughput CTC analysis system for filtration-based enrichment technologies could have profound clinical implications for improved CTC detection and analysis.

Item Type:Book Section
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Chung, Jaebum0000-0003-3932-8428
Yang, Changhuei0000-0001-8791-0354
Additional Information:© 2017 Springer Science+Business Media LLC. First Online: 18 August 2017.
Subject Keywords:Circulating tumor cells; Fourier ptychographic microscopy; Filtration-based CTC enrichment; Single-cell imaging; Metastasis
Series Name:Methods in Molecular Biology
Issue or Number:1634
Record Number:CaltechAUTHORS:20170821-084016652
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:80637
Deposited By: Tony Diaz
Deposited On:21 Aug 2017 16:19
Last Modified:15 Nov 2021 19:37

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