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Electron cryotomography

Oikonomou, C. M. and Swulius, M. T. and Briegel, A. and Beeby, M. and Yao, Q. and Chang, Y.-W. and Jensen, G. J. (2016) Electron cryotomography. In: Imaging Bacterial Molecules, Structures and Cells. Methods in Microbiology. No.43. Elsevier , Amsterdam, pp. 115-139. ISBN 9780128093924. http://resolver.caltech.edu/CaltechAUTHORS:20170825-145104411

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Abstract

Electron cryotomography (ECT) delivers 3D images of native structures inside intact cells with resolution on the scale of large macromolecular complexes (~ 4 nm). It has proven to be an invaluable tool for interrogating the organization of bacterial cells and the structures of the nanomachines inside them. Here we present a brief introduction to the technique, a few examples of what we have learned by imaging bacterial cells with ECT over the last 15 years and a frank discussion of the relative advantages and limitations of the technique compared to some other popular imaging methods used in microbiology.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/bs.mim.2016.10.001DOIArticle
http://www.sciencedirect.com/science/article/pii/S0580951716300010PublisherArticle
ORCID:
AuthorORCID
Oikonomou, C. M.0000-0003-2312-4746
Briegel, A.0000-0003-3733-3725
Beeby, M.0000-0001-6413-9835
Yao, Q.0000-0003-3575-9909
Chang, Y.-W.0000-0003-2391-473X
Jensen, G. J.0000-0003-1556-4864
Additional Information:© 2016 Elsevier Ltd. Available online 27 October 2016.
Subject Keywords:Electron cryotomography; Cryo-EM; Bacteria; Cell biology; Imaging techniques
Record Number:CaltechAUTHORS:20170825-145104411
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20170825-145104411
Official Citation:C.M. Oikonomou, M.T. Swulius, A. Briegel, M. Beeby, Q. Yao, Y.-W. Chang, G.J. Jensen, Chapter 4 - Electron cryotomography, In: Colin Harwood and Grant J. Jensen, Editor(s), Methods in Microbiology, Academic Press, 2016, Volume 43, Pages 115-139, ISSN 0580-9517, ISBN 9780128093924, https://doi.org/10.1016/bs.mim.2016.10.001. (http://www.sciencedirect.com/science/article/pii/S0580951716300010)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:80808
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:28 Aug 2017 19:56
Last Modified:23 Apr 2019 20:35

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