Oikonomou, C. M. and Swulius, M. T. and Briegel, A. and Beeby, M. and Yao, Q. and Chang, Y.-W. and Jensen, G. J. (2016) Electron cryotomography. In: Imaging Bacterial Molecules, Structures and Cells. Methods in Microbiology. No.43. Elsevier , Amsterdam, pp. 115-139. ISBN 9780128093924. https://resolver.caltech.edu/CaltechAUTHORS:20170825-145104411
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Abstract
Electron cryotomography (ECT) delivers 3D images of native structures inside intact cells with resolution on the scale of large macromolecular complexes (~ 4 nm). It has proven to be an invaluable tool for interrogating the organization of bacterial cells and the structures of the nanomachines inside them. Here we present a brief introduction to the technique, a few examples of what we have learned by imaging bacterial cells with ECT over the last 15 years and a frank discussion of the relative advantages and limitations of the technique compared to some other popular imaging methods used in microbiology.
| Item Type: | Book Section | ||||||||||||||
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| Additional Information: | © 2016 Elsevier Ltd. Available online 27 October 2016. | ||||||||||||||
| Subject Keywords: | Electron cryotomography; Cryo-EM; Bacteria; Cell biology; Imaging techniques | ||||||||||||||
| Series Name: | Methods in Microbiology | ||||||||||||||
| Issue or Number: | 43 | ||||||||||||||
| DOI: | 10.1016/bs.mim.2016.10.001 | ||||||||||||||
| Record Number: | CaltechAUTHORS:20170825-145104411 | ||||||||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20170825-145104411 | ||||||||||||||
| Official Citation: | C.M. Oikonomou, M.T. Swulius, A. Briegel, M. Beeby, Q. Yao, Y.-W. Chang, G.J. Jensen, Chapter 4 - Electron cryotomography, In: Colin Harwood and Grant J. Jensen, Editor(s), Methods in Microbiology, Academic Press, 2016, Volume 43, Pages 115-139, ISSN 0580-9517, ISBN 9780128093924, https://doi.org/10.1016/bs.mim.2016.10.001. (http://www.sciencedirect.com/science/article/pii/S0580951716300010) | ||||||||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||||||
| ID Code: | 80808 | ||||||||||||||
| Collection: | CaltechAUTHORS | ||||||||||||||
| Deposited By: | Tony Diaz | ||||||||||||||
| Deposited On: | 28 Aug 2017 19:56 | ||||||||||||||
| Last Modified: | 15 Nov 2021 19:38 |
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