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Chimeric chemosensory transducers of Escherichia coli

Krikos, Alexandra and Conley, M. Patricia and Boyd, Alan and Berg, Howard C. and Simon, Melvin I. (1985) Chimeric chemosensory transducers of Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America, 82 (5). pp. 1326-1330. ISSN 0027-8424. PMCID PMC397253. doi:10.1073/pnas.82.5.1326.

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The tar and tsr genes of Escherichia coli encode homologous transducer proteins that mediate distinct chemotactic responses. We report here the construction of two tasr chimeric genes in which the 5' coding region of the tar gene is fused to the 3' coding region of the tsr gene at either of two conserved restriction sites. Both chimeric genes code for chemotactically functional proteins. Results of analyses of behavior and methylation in cells carrying the chimeric genes support existing models for the disposition of transducer domains across the cell membrane and reveal that the receptors for internal pH map in a specific region of the COOH-terminal (cytoplasmic) domain.

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Additional Information:© 1985 by the National Academy of Sciences. Contributed by Howard C. Berg, October 22, 1984. We thank N. Mutoh, J. E. Segall, A. Ishihara, R. M. Macnab, K. Oosawa, M. F. Bruist, and E. M. Phizicky for their help. This work was supported by National Institutes of Health Grants AI16478 (to H.C.B.) and AI14988 (to M.I.S.). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
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Subject Keywords:chemotaxis; methyl-accepting chemotaxis proteins; gene family; gene fusion
Issue or Number:5
PubMed Central ID:PMC397253
Record Number:CaltechAUTHORS:KRIpnas85
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:8088
Deposited By: Tony Diaz
Deposited On:31 Jul 2007
Last Modified:08 Nov 2021 20:47

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