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A bacterial artificial chromosome-based framework contig map of human chromosome 22q

Kim, Ung-Jin and Shizuya, Hiroaki and Kang, Hyung-Lyun and Choi, Sun-Shim and Garrett, Charmain L. and Smink, Luc J. and Birren, Bruce W. and Korenberg, Julie R. and Dunham, Ian and Simon, Melvin I. (1996) A bacterial artificial chromosome-based framework contig map of human chromosome 22q. Proceedings of the National Academy of Sciences of the United States of America, 93 (13). pp. 6297-6301. ISSN 0027-8424. PMCID PMC39016. doi:10.1073/pnas.93.13.6297.

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We have constructed a physical map of human chromosome 22q using bacterial artificial chromosome (BAC) clones. The map consists of 613 chromosome 22-specific BAC clones that have been localized and assembled into contigs using 452 landmarks, 346 of which were previously ordered and mapped to specific regions of the q arm of the chromosome by means of chromosome 22-specific yeast artificial chromosome clones. The BAC-based map provides immediate access to clones that are stable and convenient for direct genome analysis. The approach to rapidly developing marker-specific BAC contigs is relatively straightforward and can be extended to generate scaffold BAC contig maps of the rest of the chromosomes. These contigs will provide substrates for sequencing the entire human genome. We discuss how to efficiently close contig gaps using the end sequences of BAC clone inserts.

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Additional Information:Copyright © 1996 by the National Academy of Sciences Contributed by Melvin I. Simon, February 26, 1996. We thank Simon Foote, Thomas Hudson, and Eric Lander for providing us with dozens of chromosome 22-specific STS primers, Charles Auffrey and Greg Lennon for chromosome 22-specific cDNAs, Jan Dumanski for KI probes, John Collins for comments on the manuscript, Ulrich Weier for the P1 probe, and Tatiana Slepak, April Mengos, and Daniel Eckstein for excellent technical assistance. This work was supported by the U.S. Department of Energy Grant FG0389ER60891. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funding AgencyGrant Number
Department of Energy (DOE)FG0389ER60891
Issue or Number:13
PubMed Central ID:PMC39016
Record Number:CaltechAUTHORS:KIMpnas96
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:809
Deposited By: Archive Administrator
Deposited On:07 Oct 2005
Last Modified:08 Nov 2021 19:05

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