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High-potential states of blue and purple copper proteins

Wittung-Stafshede, Pernilla and Gomez, Ester and Öhman, Anna and Aasa, Roland and Villahermosa, Randy M. and Leckner, Johan and Karlsson, B. Göran and Sanders, Donita and Fee, James A. and Winkler, Jay R. and Malmström, Bo G. and Gray, Harry B. and Hill, Michael G. (1998) High-potential states of blue and purple copper proteins. Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1388 (2). pp. 437-443. ISSN 0167-4838. http://resolver.caltech.edu/CaltechAUTHORS:20170830-081553147

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Abstract

Electrochemical measurements show that there are high-potential states of two copper proteins, Pseudomonas aeruginosa azurin and Thermus thermophilus Cu_A domain; these perturbed states are formed in guanidine hydrochloride (GuHCl) solution in which the proteins are still blue (azurin) and purple (Cu_A). In each case, the high-potential state forms reversibly. Absorption (azurin, Cu_A), visible circular dichroism (azurin, Cu_A), resonance-Raman (Cu_A), and EPR (Cu_A) spectra indicate that the structure of the oxidized copper site of each high-potential form is very similar to that of the native protein. It is proposed that GuHCl perturbs one or more H-bonds in the blue or purple copper active site, thereby allowing Cu(I) to adopt a more favorable coordination structure than that in the rigid cavity of the native protein.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/S0167-4838(98)00205-2DOIArticle
http://www.sciencedirect.com/science/article/pii/S0167483898002052PublisherArticle
ORCID:
AuthorORCID
Wittung-Stafshede, Pernilla0000-0003-1058-1964
Winkler, Jay R.0000-0002-4453-9716
Gray, Harry B.0000-0002-7937-7876
Additional Information:© 1998 Elsevier Science B.V. Received 27 April 1998, Revised 30 July 1998, Accepted 19 August 1998, Available online 20 April 1999. P.W.-S. thanks the Swedish Technical Research Council for a postdoctoral fellowship. This investigation was supported by NSF, NIH, the Arnold and Mabel Beckman Foundation, the Nobel Committee for Chemistry (B.G.M.), the Swedish Natural Science Research Council (B.G.K.), and the Research Corporation (Cottrell Science Award to M.G.H.).
Funders:
Funding AgencyGrant Number
Swedish Technical Research CouncilUNSPECIFIED
NSFUNSPECIFIED
NIHUNSPECIFIED
Arnold and Mabel Beckman FoundationUNSPECIFIED
Nobel Committee for ChemistryUNSPECIFIED
Swedish Natural Science Research CouncilUNSPECIFIED
Cottrell Scholar of Research CorporationUNSPECIFIED
Subject Keywords:Azurin; Copper-A; Electrochemistry; High-potential protein
Record Number:CaltechAUTHORS:20170830-081553147
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20170830-081553147
Official Citation:Pernilla Wittung-Stafshede, Ester Gomez, Anna Öhman, Roland Aasa, Randy M. Villahermosa, Johan Leckner, B.Göran Karlsson, Donita Sanders, James A. Fee, Jay R. Winkler, Bo G. Malmström, Harry B. Gray, Michael G. Hill, High-potential states of blue and purple copper proteins, Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, Volume 1388, Issue 2, 10 November 1998, Pages 437-443, ISSN 0167-4838, https://doi.org/10.1016/S0167-4838(98)00205-2. (http://www.sciencedirect.com/science/article/pii/S0167483898002052)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:80944
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:30 Aug 2017 16:07
Last Modified:12 Jul 2019 22:46

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