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Incubator embedded cell culture imaging system (EmSight) based on Fourier ptychographic microscopy

Kim, Jinho and Henley, Beverley M. and Kim, Charlene H. and Lester, Henry A. and Yang, Changhuei (2017) Incubator embedded cell culture imaging system (EmSight) based on Fourier ptychographic microscopy. In: Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV. Proceedings of SPIE. No.10068. Society of Photo-Optical Instrumentation Engineers , Bellingham, WA, Art. No. 100680X. ISBN 9781510605770. https://resolver.caltech.edu/CaltechAUTHORS:20170831-081004023

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Abstract

Multi-day tracking of cells in culture systems can provide valuable information in bioscience experiments. We report the development of a cell culture imaging system, named EmSight, which incorporates multiple compact Fourier ptychographic microscopes with a standard multiwell imaging plate. The system is housed in an incubator and presently incorporates six microscopes, imaging an ANSI standard 6-well plate at the same time. By using the same low magnification objective lenses (NA of 0.1) as the objective and the tube lens, the EmSight is configured as a 1:1 imaging system that, providing large field-of-view (FOV) imaging (5.7 mm × 4.3 mm) onto a low-cost CMOS imaging sensor. The EmSight improves the image resolution by capturing a series of images of the sample at varying illumination angles; the instrument reconstructs a higher-resolution image by using the iterative Fourier ptychographic algorithm. In addition to providing high-resolution brightfield and phase imaging, the EmSight is also capable of fluorescence imaging at the native resolution of the objectives. We characterized the system using a phase Siemens star target, and show four-fold improved coherent resolution (synthetic NA of 0.42) and a depth of field of 0.2 mm. To conduct live, long-term dopaminergic neuron imaging, we cultured ventral midbrain from mice driving eGFP from the tyrosine hydroxylase promoter. The EmSight system tracks movements of dopaminergic neurons over a 21 day period.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1117/12.2249906.5380018861001DOIPresentation
http://videos.spie.org/services/player/bcpid1094074693001?bckey=AQ~~,AAAAoHrMRhk~,T5-k00gMv_uqr7ekZHfpqOsmv-JJJWhw&bctid=5380018861001PublisherPresentation
http://resolver.caltech.edu/CaltechAUTHORS:20160801-135931634Related ItemArticle
ORCID:
AuthorORCID
Lester, Henry A.0000-0002-5470-5255
Additional Information:© 2017 Society of Photo-Optical Instrumentation Engineers (SPIE).
Series Name:Proceedings of SPIE
Issue or Number:10068
Record Number:CaltechAUTHORS:20170831-081004023
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170831-081004023
Official Citation:Jinho Kim, Beverley M. Henley, Charlene H. Kim, Henry A. Lester, Changhuei Yang, "Incubator embedded cell culture imaging system (EmSight) based on Fourier ptychographic microscopy (Conference Presentation)," Proc. SPIE 10068, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV, 100680X;
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:81011
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:31 Aug 2017 15:52
Last Modified:03 Oct 2019 18:37

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