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Transfection of Folate-Polylysine DNA Complexes: Evidence for Lysosomal Delivery

Mislick, Kimberly A. and Baldeschwieler, John D. and Kayyem, Jon F. and Meade, Thomas J. (1995) Transfection of Folate-Polylysine DNA Complexes: Evidence for Lysosomal Delivery. Bioconjugate Chemistry, 6 (5). pp. 512-515. ISSN 1043-1802. doi:10.1021/bc00035a002.

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We are utilizing the folate receptor for the intracellular delivery of DNA. In this study, a folate-poly-L-lysine (FPLL) conjugate was synthesized and equilibrated with plasmid DNA encoding the firefly luciferase gene. The FPLL-DN_A complexes were added to KB cells treated with chloroquine. Luciferase activity of cells incubated with FPLL-DNA was 6-fold higher than of cells exposed to poly-L-lysine. (PLL)-DNA. The addition of free folic acid competitively inhibited the enhancement of gene expression. Removal of chloroquine from the media significantly inhibited transfection efficiency of FPLL-DNA complexes. We conclude that FPLL-DNA complexes are delivered into KB cells via folate receptor-mediated endocytosis and likely follow a lysosomal pathway into the cytoplasm.

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Additional Information:© 1995 American Chemical Society. Received June 15, 1995. This work was funded in part by a USPHS Training Grant No. GM08346, Nexstar, Inc., and the Biological Imaging Center. We thank Dr. Rex Moats and Dr. Wilton Vannier for helpful discussions.
Funding AgencyGrant Number
NIH Predoctoral FellowshipGM08346
Issue or Number:5
Record Number:CaltechAUTHORS:20170905-095408869
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Official Citation:Transfection of Folate-Polylysine DNA Complexes: Evidence for Lysosomal Delivery Kimberly A. Mislick, John D. Baldeschwieler, Jon F. Kayyem, and Thomas J. Meade Bioconjugate Chemistry 1995 6 (5), 512-515 DOI: 10.1021/bc00035a002
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:81128
Deposited By: Tony Diaz
Deposited On:05 Sep 2017 19:34
Last Modified:15 Nov 2021 19:41

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