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Transfection of Folate-Polylysine DNA Complexes: Evidence for Lysosomal Delivery

Mislick, Kimberly A. and Baldeschwieler, John D. and Kayyem, Jon F. and Meade, Thomas J. (1995) Transfection of Folate-Polylysine DNA Complexes: Evidence for Lysosomal Delivery. Bioconjugate Chemistry, 6 (5). pp. 512-515. ISSN 1043-1802. https://resolver.caltech.edu/CaltechAUTHORS:20170905-095408869

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Abstract

We are utilizing the folate receptor for the intracellular delivery of DNA. In this study, a folate-poly-L-lysine (FPLL) conjugate was synthesized and equilibrated with plasmid DNA encoding the firefly luciferase gene. The FPLL-DN_A complexes were added to KB cells treated with chloroquine. Luciferase activity of cells incubated with FPLL-DNA was 6-fold higher than of cells exposed to poly-L-lysine. (PLL)-DNA. The addition of free folic acid competitively inhibited the enhancement of gene expression. Removal of chloroquine from the media significantly inhibited transfection efficiency of FPLL-DNA complexes. We conclude that FPLL-DNA complexes are delivered into KB cells via folate receptor-mediated endocytosis and likely follow a lysosomal pathway into the cytoplasm.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1021/bc00035a002DOIArticle
http://pubs.acs.org/doi/abs/10.1021/bc00035a002PublisherArticle
Additional Information:© 1995 American Chemical Society. Received June 15, 1995. This work was funded in part by a USPHS Training Grant No. GM08346, Nexstar, Inc., and the Biological Imaging Center. We thank Dr. Rex Moats and Dr. Wilton Vannier for helpful discussions.
Funders:
Funding AgencyGrant Number
NIH Predoctoral FellowshipGM08346
Nexstar, Inc.UNSPECIFIED
Issue or Number:5
Record Number:CaltechAUTHORS:20170905-095408869
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170905-095408869
Official Citation:Transfection of Folate-Polylysine DNA Complexes: Evidence for Lysosomal Delivery Kimberly A. Mislick, John D. Baldeschwieler, Jon F. Kayyem, and Thomas J. Meade Bioconjugate Chemistry 1995 6 (5), 512-515 DOI: 10.1021/bc00035a002
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:81128
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:05 Sep 2017 19:34
Last Modified:03 Oct 2019 18:39

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