Mislick, Kimberly A. and Baldeschwieler, John D. and Kayyem, Jon F. and Meade, Thomas J. (1995) Transfection of Folate-Polylysine DNA Complexes: Evidence for Lysosomal Delivery. Bioconjugate Chemistry, 6 (5). pp. 512-515. ISSN 1043-1802. doi:10.1021/bc00035a002. https://resolver.caltech.edu/CaltechAUTHORS:20170905-095408869
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Abstract
We are utilizing the folate receptor for the intracellular delivery of DNA. In this study, a folate-poly-L-lysine (FPLL) conjugate was synthesized and equilibrated with plasmid DNA encoding the firefly luciferase gene. The FPLL-DN_A complexes were added to KB cells treated with chloroquine. Luciferase activity of cells incubated with FPLL-DNA was 6-fold higher than of cells exposed to poly-L-lysine. (PLL)-DNA. The addition of free folic acid competitively inhibited the enhancement of gene expression. Removal of chloroquine from the media significantly inhibited transfection efficiency of FPLL-DNA complexes. We conclude that FPLL-DNA complexes are delivered into KB cells via folate receptor-mediated endocytosis and likely follow a lysosomal pathway into the cytoplasm.
| Item Type: | Article | |||||||||
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| Additional Information: | © 1995 American Chemical Society. Received June 15, 1995. This work was funded in part by a USPHS Training Grant No. GM08346, Nexstar, Inc., and the Biological Imaging Center. We thank Dr. Rex Moats and Dr. Wilton Vannier for helpful discussions. | |||||||||
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| Issue or Number: | 5 | |||||||||
| DOI: | 10.1021/bc00035a002 | |||||||||
| Record Number: | CaltechAUTHORS:20170905-095408869 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20170905-095408869 | |||||||||
| Official Citation: | Transfection of Folate-Polylysine DNA Complexes: Evidence for Lysosomal Delivery Kimberly A. Mislick, John D. Baldeschwieler, Jon F. Kayyem, and Thomas J. Meade Bioconjugate Chemistry 1995 6 (5), 512-515 DOI: 10.1021/bc00035a002 | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 81128 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Tony Diaz | |||||||||
| Deposited On: | 05 Sep 2017 19:34 | |||||||||
| Last Modified: | 15 Nov 2021 19:41 |
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