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Cross-Linking Exogenous Bifunctional Peptides into Fibrin Gels with Factor XIIIa

Schense, Jason C. and Hubbell, Jeffrey A. (1999) Cross-Linking Exogenous Bifunctional Peptides into Fibrin Gels with Factor XIIIa. Bioconjugate Chemistry, 10 (1). pp. 75-81. ISSN 1043-1802. doi:10.1021/bc9800769.

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Bi-domain peptides with a factor XIIIa substrate in one domain and a bioactive peptide in another domain were covalently incorporated into fibrin gels during coagulation through the action of the transglutaminase factor XIIIa. The cross-linking characteristics were determined for two bi-domain peptides with factor XIIIa substrates based on fibrinogen, dYRGDTIGEGQQHHLGG-NH_2, and dLRGDGAKDV-NH_2, as well as one bi-domain peptide with a substrate sequence based on α_2-plasmin inhibitor, dLNQEQVSPLRGD-NH_2, and another with a nonbiological, oligolysine substrate, dLRGDKKKKG-NH_2 (substrate domains in italic). Each of these peptides was able to cross-link into the fibrin gels during coagulation, with the peptide containing the factor XIIIa substrate based on α2-plasmin inhibitor being incorporated at levels in excess of 8 mol/mol fibrinogen. The structural characteristics of these peptide-modified gels proved to be the same as those for a native fibrin gel. The bioactivity of the incorporated active factors was tested in a neuronal culture model with day 8 chicken dorsal root ganglia using two bioactive sequences, RGD and DGEA, and one inactive control sequence, RDG. Each of these peptides influenced the extension of neurites from the ganglia as expected, indicating that the incorporated factors retained their activity. With the use of soluble competitive inhibitors, it was shown that this effect was due to the covalently incorporated peptides. Through exploiting the role of factor XIIIa in coagulation, we have developed a method by which to impart the character of nonfibrin proteins, such as extracellular matrix proteins, to fibrin, a biological material with many potential therapeutic and academic applications.

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Additional Information:© 1999 American Chemical Society. Received June 26, 1998; Revised Manuscript Received October 13, 1998. This work was funded by NIH Predoctoral Training in Biotechnology, Grant GM08346, and by Grant 31-52261 NFP 38 of the Swiss National Science Foundation.
Funding AgencyGrant Number
NIH Predoctoral FellowshipGM08346
Swiss National Science Foundation (SNSF)31-52261 NFP 38
Issue or Number:1
Record Number:CaltechAUTHORS:20170905-100818026
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Official Citation:Cross-Linking Exogenous Bifunctional Peptides into Fibrin Gels with Factor XIIIa Jason C. Schense and Jeffrey A. Hubbell Bioconjugate Chemistry 1999 10 (1), 75-81 DOI: 10.1021/bc9800769
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:81130
Deposited By: Tony Diaz
Deposited On:05 Sep 2017 19:31
Last Modified:15 Nov 2021 19:41

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