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Protein-Mediated Colloidal Assembly

Obana, Maiko and Silverman, Bradley R. and Tirrell, David A. (2017) Protein-Mediated Colloidal Assembly. Journal of the American Chemical Society, 139 (40). pp. 14251-14256. ISSN 0002-7863. PMCID PMC5672941.

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Programmable colloidal assembly enables the creation of mesoscale materials in a bottom-up manner. Although DNA oligonucleotides have been used extensively as the programmable units in this paradigm, proteins, which exhibit more diverse modes of association and function, have not been widely used to direct colloidal assembly. Here we use protein–protein interactions to drive controlled aggregation of polystyrene microparticles, either through reversible coiled-coil interactions or through intermolecular isopeptide linkages. The sizes of the resulting aggregates are tunable and can be controlled by the concentration of immobilized surface proteins. Moreover, particles coated with different protein pairs undergo orthogonal assembly. We demonstrate that aggregates formed by association of coiled-coil proteins, in contrast to those linked by isopeptide bonds, are dispersed by treatment with chemical denaturants or soluble competing proteins. Finally, we show that protein–protein interactions can be used to assemble complex core–shell aggregates. This work illustrates a versatile strategy for engineering colloidal systems for use in materials science and biotechnology.

Item Type:Article
Related URLs:
URLURL TypeDescription Information CentralArticle
Silverman, Bradley R.0000-0002-9256-8941
Tirrell, David A.0000-0003-3175-4596
Additional Information:© 2017 American Chemical Society. Received: August 1, 2017; Published: September 12, 2017. We thank Trudy Padmore for technical assistance with particle conjugation and Andres Collazo for assistance with confocal microscopy. This work was supported by Defense Advanced Research Projects Agency Biological Robustness in Complex Settings Contract HR001-15-C-0093. M.O is supported by the Nakajima Foundation. B.S. is supported by NIH Training Grant 1T32GM112592 and by the Rosen Center for Bioengineering. Imaging was performed in the Biological Imaging Facility, with the support of the Caltech Beckman Institute and the Arnold and Mabel Beckman Foundation. Author Contributions: M.O and B.R.S. contributed equally to this work. The authors declare the absence of any competing financial interests.
Group:Rosen Bioengineering Center
Funding AgencyGrant Number
Defense Advanced Research Projects Agency (DARPA)HR001-15-C-0093
Nakajima FoundationUNSPECIFIED
NIH Predoctoral Fellowship1T32GM112592
Donna and Benjamin M. Rosen Bioengineering CenterUNSPECIFIED
Caltech Beckman InstituteUNSPECIFIED
Arnold and Mabel Beckman FoundationUNSPECIFIED
Issue or Number:40
PubMed Central ID:PMC5672941
Record Number:CaltechAUTHORS:20170913-101124804
Persistent URL:
Official Citation:Protein-Mediated Colloidal Assembly Maiko Obana, Bradley R. Silverman, and David A. Tirrell Journal of the American Chemical Society 2017 139 (40), 14251-14256 DOI: 10.1021/jacs.7b07798
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:81405
Deposited By: Tony Diaz
Deposited On:13 Sep 2017 22:36
Last Modified:09 Mar 2020 13:19

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