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Deconstruction of the beaten Path-Sidestep interaction network provides insights into neuromuscular system development

Li, Hanqing and Watson, Ash and Olechwier, Agnieszka and Anaya, Michael and Sorooshyari, Siamak K. and Harnett, Dermott P. and Lee, Hyung-Kook (Peter) and Vielmetter, Jost and Fares, Mario A. and Garcia, K. Christopher and Özkan, Engin and Labrador, Juan-Pablo and Zinn, Kai (2017) Deconstruction of the beaten Path-Sidestep interaction network provides insights into neuromuscular system development. eLife, 6 . Art. No. e28111. ISSN 2050-084X. PMCID PMC5578738. http://resolver.caltech.edu/CaltechAUTHORS:20170914-141939632

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Abstract

An ‘interactome’ screen of all Drosophila cell-surface and secreted proteins containing immunoglobulin superfamily (IgSF) domains discovered a network formed by paralogs of Beaten Path (Beat) and Sidestep (Side), a ligand-receptor pair that is central to motor axon guidance. Here we describe a new method for interactome screening, the Bio-Plex Interactome Assay (BPIA), which allows identification of many interactions in a single sample. Using the BPIA, we ‘deorphanized’ four more members of the Beat-Side network. We confirmed interactions using surface plasmon resonance. The expression patterns of beat and side genes suggest that Beats are neuronal receptors for Sides expressed on peripheral tissues. side-VI is expressed in muscle fibers targeted by the ISNb nerve, as well as at growth cone choice points and synaptic targets for the ISN and TN nerves. beat-V genes, encoding Side-VI receptors, are expressed in ISNb and ISN motor neurons.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.7554/eLife.28111DOIArticle
https://elifesciences.org/articles/28111PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5578738PubMed CentralArticle
ORCID:
AuthorORCID
Sorooshyari, Siamak K.0000-0002-1172-6291
Garcia, K. Christopher0000-0001-9273-0278
Özkan, Engin0000-0002-0263-6729
Zinn, Kai0000-0002-6706-5605
Additional Information:© 2017 Li et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. Received: 27 April 2017; Accepted: 28 July 2017; Published: 15 August 2017. This work was supported by NIH grants to KZ (R37 NS28182), and to EÖ (RO1 NS097161), by the Klingenstein-Simons Fellowship in the Neurosciences to EÖ, and by SFI grants 07/IN.1/B913 and 08/RFP/NSC1617 to J-P L. We thank Aref Arzan Zarin for preliminary genetic analysis. We thank Elena Armand and Suzanne Fisher for technical assistance, Maria Prats for preparation of AP supernatants for the experiments of Figs. S2 and S3, and Violana Nesterova for figure preparation. We thank Kaushiki Menon and Namrata Bali for help with larval staining. We acknowledge Dr. Elena Solomaha and the University of Chicago BioPhysics Core Facilities for training with and access to a Biacore 3000. We thank Laura Quintana Rio, Lalanti Venkatasubramanian, and Richard Mann (Columbia) for the Side-VI-T2A-GAL4 line. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. Author Contributions: Hanqing Li, Conceptualization, Resources, Data curation, Formal analysis, Validation, Investigation, Writing—original draft, Writing—review and editing; Ash Watson, Hyung-Kook (Peter) Lee, Investi- gation, Visualization; Agnieszka Olechwier, Michael Anaya, Validation, Investigation, Methodology; Siamak K Sorooshyari, Software, Investigation, Methodology; Dermott P Harnett, Investigation, Methodology; Jost Vielmetter, Resources, Data curation, Funding acquisition, Validation, Investiga- tion, Methodology, Project administration; Mario A Fares, Conceptualization, Software, Investiga- tion, Methodology; K Christopher Garcia, Conceptualization, Resources, Project administration; Engin O¨ zkan, Conceptualization, Data curation, Formal analysis, Supervision, Funding acquisition, Validation, Investigation, Visualization, Methodology, Project administration, Writing—review and editing; Juan-Pablo Labrador, Conceptualization, Resources, Data curation, Formal analysis, Supervision, Funding acquisition, Validation, Investigation, Visualization, Methodology, Project administration, Writing—review and editing; Kai Zinn, Conceptualization, Data curation, Formal analysis, Supervision, Funding acquisition, Investigation, Visualization, Methodology, Writing—original draft, Project administration, Writing—review and editing
Funders:
Funding AgencyGrant Number
NIHR37 NS28182
NIHRO1 NS097161
Klingenstein-Simons FellowshipUNSPECIFIED
Science Foundation, Ireland07/IN.1/B913
Science Foundation, Ireland08/RFP/NSC1617
PubMed Central ID:PMC5578738
Record Number:CaltechAUTHORS:20170914-141939632
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20170914-141939632
Official Citation:Li, H., Watson, A., Olechwier, A., Anaya, M., Sorooshyari, S. K., Harnett, D. P., … Zinn, K. (2017). Deconstruction of the beaten Path-Sidestep interaction network provides insights into neuromuscular system development. eLife, 6, e28111. http://doi.org/10.7554/eLife.28111
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:81454
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:14 Sep 2017 21:39
Last Modified:14 Sep 2017 21:39

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