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Correcting the limited view in optical-resolution photoacoustic microscopy

Liu, Wei and Zhou, Yuan and Wang, Mengran and Li, Lei and Vienneau, Emelina and Chen, Ruimin and Luo, Jianwen and Xu, Chris and Zhou, Qifa and Wang, Lihong V. and Yao, Junjie (2018) Correcting the limited view in optical-resolution photoacoustic microscopy. Journal of Biophotonics, 11 (2). Art. No. e201700196. ISSN 1864-063X. doi:10.1002/jbio.201700196.

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Optical-resolution photoacoustic microscopy (OR-PAM) has proven useful for anatomical and functional imaging with high spatial resolutions. However, the coherent signal generation and the desired reflection-mode detection in OR-PAM can result in a limited detectability of features aligned with the acoustic axis (i.e., vertical structures). Here, we investigated the limited-view phenomenon in OR-PAM by simulating the generation and propagation of the acoustic pressure waves and determined the key optical parameters affecting the visibility of vertical structures. Proof-of-concept numerical experiments were performed with different illumination angles, optical foci, and numerical apertures (NA) of the objective lens. The results collectively show that an NA of 0.3 can readily improve the visibility of vertical structures in a typical reflection-mode OR-PAM system. This conclusion was confirmed by numerical simulations on the cortical blood vessels in a mouse brain, and by experiments in a suture-cross phantom and in a mouse brain in vivo.

Item Type:Article
Related URLs:
URLURL TypeDescription
Liu, Wei0000-0002-1727-6738
Li, Lei0000-0001-6164-2646
Chen, Ruimin0000-0002-5338-359X
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2017 Wiley. Accepted manuscript online: 3 October 2017; Manuscript Accepted: 27 September 2017; Manuscript Revised: 14 September 2017; Manuscript Received: 27 July 2017. Funded by: Duke MEDx.
Funding AgencyGrant Number
Subject Keywords:photoacoustic imaging; limited view; mouse brain; k-wave; coherent signal
Issue or Number:2
Record Number:CaltechAUTHORS:20171004-083525247
Persistent URL:
Official Citation:Liu W, Zhou Y, Wang M, et al. Correcting the limited view in optical-resolution photoacoustic microscopy. J. Biophotonics. 2018;11:e201700196.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:82031
Deposited By: Tony Diaz
Deposited On:04 Oct 2017 16:58
Last Modified:15 Nov 2021 19:47

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