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Composing RNA nanostructures from a syntax of RNA structural modules

Geary, Cody and Chworos, Arkadiusz and Verzemnieks, Erik and Voss, Neil R. and Jaeger, Luc (2017) Composing RNA nanostructures from a syntax of RNA structural modules. Nano Letters, 17 (11). pp. 7095-7101. ISSN 1530-6984. http://resolver.caltech.edu/CaltechAUTHORS:20171017-105425672

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Abstract

Natural stable RNAs fold and assemble into complex three-dimensional architectures by relying on the hierarchical formation of intricate, recurrent networks of noncovalent tertiary interactions. These sequence-dependent networks specify RNA structural modules enabling orientational and topological control of helical struts to form larger self-folding domains. Borrowing concepts from linguistics, we defined an extended structural syntax of RNA modules for programming RNA strands to assemble into complex, responsive nanostructures under both thermodynamic and kinetic control. Based on this syntax, various RNA building blocks promote the multimolecular assembly of objects with well-defined three-dimensional shapes as well as the isothermal folding of long RNAs into complex single-stranded nanostructures during transcription. This work offers a glimpse of the limitless potential of RNA as an informational medium for designing programmable and functional nanomaterials useful for synthetic biology, nanomedicine, and nanotechnology.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1021/acs.nanolett.7b03842DOIArticle
http://pubs.acs.org/doi/abs/10.1021/acs.nanolett.7b03842PublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/acs.nanolett.7b03842PublisherSupporting Information
ORCID:
AuthorORCID
Geary, Cody0000-0003-2083-4259
Jaeger, Luc0000-0001-9068-348X
Additional Information:© 2017 American Chemical Society. Received: September 7, 2017; Revised: October 15, 2017; Published: October 17, 2017. L.J. thanks Maria del Carmen Jaeger for her silent contributions during the 12 years of this project. The authors thank E. Jacovetty, C. Potter, and B. Carragher for their scientific input regarding cryo-EM and single-particle reconstruction. Cryo-EM was performed at the National Resource for Automated Molecular Microscopy, which is supported by the NIH National Institute of General Medical Sciences (grant no. GM103310). This work was supported by the National Institutes of Health (grant nos. R01GM079604 and 5P41RR017573-10 to L.J.) and intramural research grants from the UCSB Academic Senate (to LJ). C.G. acknowledges support from U.S. National Science Foundation Expedition in Computing (grant no. 1317694) and support from the Carlsberg Foundation. This paper is dedicated to the Three Hearts of the Holy Family in memory of Prof. Albert Jaeger, MD. Author Contributions: L.J. and C.G. conceived and designed experiments; C.G., A.C., and E.V. performed biochemical experiments; A.C. and C.G. performed AFM; N.V. performed cryo-EM: L.J. wrote the paper with the help of C.G. and A.C. The authors declare no competing financial interest.
Funders:
Funding AgencyGrant Number
NIHGM103310
NIHR01GM079604
NIH5P41RR017573-10
University of California, Santa BarbaraUNSPECIFIED
NSFCCF-1317694
Carlsberg FoundationUNSPECIFIED
Subject Keywords:RNA nanotechnology, RNA self-assembly, RNA folding, nanostructures, nanoparticles, tectoRNAs, RNA
Record Number:CaltechAUTHORS:20171017-105425672
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20171017-105425672
Official Citation:Composing RNA Nanostructures from a Syntax of RNA Structural Modules. Cody Geary, Arkadiusz Chworos, Erik Verzemnieks, Neil R. Voss, and Luc Jaeger. Nano Letters 2017 17 (11), 7095-7101. DOI: 10.1021/acs.nanolett.7b03842
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:82414
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:17 Oct 2017 21:23
Last Modified:22 Apr 2019 23:51

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