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Genetic Diversity and Potential Function of Microbial Symbionts Associated with Newly Discovered Species of Osedax Polychaete Worms

Goffredi, Shana K. and Johnson, Shannon B. and Vrijenhoek, Robert C. (2007) Genetic Diversity and Potential Function of Microbial Symbionts Associated with Newly Discovered Species of Osedax Polychaete Worms. Applied and Environmental Microbiology, 73 (7). pp. 2314-2323. ISSN 0099-2240. PMCID PMC1855680. doi:10.1128/AEM.01986-06. https://resolver.caltech.edu/CaltechAUTHORS:GOFaem07

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Abstract

We investigated the genetic diversity of symbiotic bacteria associated with two newly discovered species of Osedax from Monterey Canyon, CA, at 1,017-m (Osedax Monterey Bay sp. 3 "rosy" [Osedax sp. MB3]) and 381-m (Osedax Monterey Bay sp. 4 "yellow collar") depths. Quantitative PCR and clone libraries of 16S rRNA gene sequences identified differences in the compositions and abundances of bacterial phylotypes associated with the newly discovered host species and permitted comparisons between adult Osedax frankpressi and juveniles that had recently colonized whalebones implanted at 2,891 m. The newly discovered Osedax species hosted Oceanospirillales symbionts that are related to Gammaproteobacteria associated with the previously described O. frankpressi and Osedax rubiplumus (S. K. Goffredi, V. J. Orphan, G. W. Rouse, L. Jahnke, T. Embaye, K. Turk, R. Lee, and R. C. Vrijenhoek, Environ. Microbiol. 7:1369-1378, 2005). In addition, Osedax sp. MB3 hosts a diverse and abundant population of additional bacteria dominated by Epsilonproteobacteria. Ultrastructural analysis of symbiont-bearing root tissues verified the enhanced microbial diversity of Osedax sp. MB3. Root tissues from the newly described host species and O. frankpressi all exhibited collagenolytic enzyme activity, which covaried positively with the abundance of symbiont DNA and negatively with mean adult size of the host species. Members of this unusual genus of bone-eating worms may form variable associations with symbiotic bacteria that allow for the observed differences in colonization and success in whale fall environments throughout the world's oceans.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1128/AEM.01986-06DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1855680/PubMed CentralArticle
ORCID:
AuthorORCID
Goffredi, Shana K.0000-0002-9110-9591
Additional Information:© 2007, American Society for Microbiology. Received 23 August 2006/ Accepted 20 January 2007. Published ahead of print on 2 February 2007. This work was supported by grants from the David and Lucile Packard Foundation, a Davidow grant to Caltech's Geological and Planetary Sciences division, and the U.S. National Science Foundation (MCB-0454860 to S.K.G.). We thank the Tiburon pilots and Western Flyer crew, W. J. Jones for laboratory and shipboard support, R. Young for help with phylogenetic analyses, A. Pernthaler for advice regarding FISH analyses, J. Leadbetter for laboratory space at the California Institute of Technology, and V. Orphan for use of both sequencing and microscopy facilities (also at Caltech).
Issue or Number:7
PubMed Central ID:PMC1855680
DOI:10.1128/AEM.01986-06
Record Number:CaltechAUTHORS:GOFaem07
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:GOFaem07
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:8286
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:01 Aug 2007
Last Modified:08 Nov 2021 20:49

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