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Consumption of Methane and CO_2 by Methanotrophic Microbial Mats from Gas Seeps of the Anoxic Black Sea

Treude, Tina and Orphan, Victoria J. and Knittel, Katrin and Gieseke, Armin and House, Christopher H. and Boetius, Antje (2007) Consumption of Methane and CO_2 by Methanotrophic Microbial Mats from Gas Seeps of the Anoxic Black Sea. Applied and Environmental Microbiology, 73 (7). pp. 2271-2283. ISSN 0099-2240. PMCID PMC1855681. https://resolver.caltech.edu/CaltechAUTHORS:TREaem07

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Abstract

The deep anoxic shelf of the northwestern Black Sea has numerous gas seeps, which are populated by methanotrophic microbial mats in and above the seafloor. Above the seafloor, the mats can form tall reef-like structures composed of porous carbonate and microbial biomass. Here, we investigated the spatial patterns of CH_4 and CO_2 assimilation in relation to the distribution of ANME groups and their associated bacteria in mat samples obtained from the surface of a large reef structure. A combination of different methods, including radiotracer incubation, beta microimaging, secondary ion mass spectrometry, and catalyzed reporter deposition fluorescence in situ hybridization, was applied to sections of mat obtained from the large reef structure to locate hot spots of methanotrophy and to identify the responsible microbial consortia. In addition, CO_2 reduction to methane was investigated in the presence or absence of methane, sulfate, and hydrogen. The mat had an average δ^(13)C carbon isotopic signature of −67.1‰, indicating that methane was the main carbon source. Regions dominated by ANME-1 had isotope signatures that were significantly heavier (−66.4‰ ± 3.9 ‰ [mean ± standard deviation; n = 7]) than those of the more central regions dominated by ANME-2 (−72.9‰ ± 2.2 ‰; n = 7). Incorporation of ^(14)C from radiolabeled CH_4 or CO_2 revealed one hot spot for methanotrophy and CO2 fixation close to the surface of the mat and a low assimilation efficiency (1 to 2% of methane oxidized). Replicate incubations of the mat with ^(14)CH_4 or ^(14)CO_2 revealed that there was interconversion of CH_4 and CO_2. The level of CO_2 reduction was about 10% of the level of anaerobic oxidation of methane. However, since considerable methane formation was observed only in the presence of methane and sulfate, the process appeared to be a rereaction of anaerobic oxidation of methane rather than net methanogenesis.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1855681/PubMed CentralArticle
http://aem.asm.org/content/73/11/3770DOIErrata
ORCID:
AuthorORCID
Orphan, Victoria J.0000-0002-5374-6178
Alternate Title:Consumption of Methane and CO2 by Methanotrophic Microbial Mats from Gas Seeps of the Anoxic Black Sea
Additional Information:Copyright © 2007, American Society for Microbiology. Received 16 November 2006/ Accepted 20 January 2007. Published ahead of print on 2 February 2007. We acknowledge F. Widdel for his advice concerning the interpretation of the methane formation experiments. We thank K. Nauhaus and N. Finke for fruitful discussions about methane formation in the mat. We thank the officers, crew, and shipboard scientific party of R/V Professor Logachev and the JAGO Team for excellent support during the Black Sea cruise in summer 2001. Special thanks are due to W. Michaelis. We acknowledge B. Ratunde, T. Wilkop, and I. Mueller for providing technical assistance. We thank the GHOSTDABS project and the JAGO Team for providing the reef pictures. We thank Kevin McKeegan for assistance with the SIMS configuration. Two anonymous reviewers are acknowledged for their comments. The UCLA ion microprobe is supported by the W.M. Keck Foundation and by a grant from the National Science Foundation Instrumentation and Facilities Program (grant EAR 01-13563). Support for V. Orphan and C. House, as well as for the SIMS analyses, was provided by the NSF Microbial Interactions and Processes Program (grant 03488492). This is a publication of the MUMM (03G0554A) and GHOSTDABS (03G0559A) programs supported by the German Ministry of Education and Research (BMBF) and the German Research Foundation (DFG). Additional support was provided by the Max-Planck-Gesellschaft (Germany) and by the NASA Astrobiology Institute. Publication no. GEOTECH-258 of the R&D program GEOTECHNOLOGIEN.
Errata:Erratum for Treude et al., Appl. Environ. Microbiol. 73 (7) 2271-2283. Applied and Environmental Microbiology, June 2007, p. 3770, Vol. 73, No. 11 doi:10.1128/AEM.00806-07
Funders:
Funding AgencyGrant Number
W. M. Keck FoundationUNSPECIFIED
NSFEAR 01-13563
Bundesministerium für Bildung und Forschung (BMBF)UNSPECIFIED
Deutsche Forschungsgemeinschaft (DFG)UNSPECIFIED
Max-Planck-GesellschaftUNSPECIFIED
NASAUNSPECIFIED
Issue or Number:7
PubMed Central ID:PMC1855681
Record Number:CaltechAUTHORS:TREaem07
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:TREaem07
Alternative URL:http://dx.doi.org/10.1128/AEM.02685-06
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:8287
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:01 Aug 2007
Last Modified:02 Oct 2019 23:49

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