Tycko, Josh and Van, Mike V. and Elowitz, Michael B. and Bintu, Lacramioara (2017) Advancing towards a global mammalian gene regulation model through single-cell analysis and synthetic biology. Current Opinion in Biomedical Engineering, 4 . pp. 174-193. ISSN 2468-4511. doi:10.1016/j.cobme.2017.10.011. https://resolver.caltech.edu/CaltechAUTHORS:20171114-145510367
Full text is not posted in this repository. Consult Related URLs below.
Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20171114-145510367
Abstract
Engineering complex genetic functions in mammalian cells will require predictive models of gene regulation. Since gene expression is stochastic, leading to cell-to-cell heterogeneity, these models depend on single-cell measurements. Here, we summarize recent microscopy and sequencing-based single-cell measurements of transcription and its chromatin-based regulation. Then, we describe synthetic biology methods for manipulating chromatin, and highlight how they could be coupled to single-cell measurements. We discuss theoretical models that connect some chromatin inputs to transcriptional outputs. Finally, we point out the connections between the models that would allow us to integrate them into one global input-output gene regulatory function.
Item Type: | Article | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Related URLs: |
| |||||||||
ORCID: |
| |||||||||
Additional Information: | © 2017 Published by Elsevier Inc. Received 25 July 2017, Revised 4 October 2017, Accepted 26 October 2017, Available online 10 November 2017. | |||||||||
DOI: | 10.1016/j.cobme.2017.10.011 | |||||||||
Record Number: | CaltechAUTHORS:20171114-145510367 | |||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20171114-145510367 | |||||||||
Official Citation: | Josh Tycko, Mike V. Van, Michael B. Elowitz, Lacramioara Bintu, Advancing towards a global mammalian gene regulation model through single-cell analysis and synthetic biology, In Current Opinion in Biomedical Engineering, Volume 4, 2017, Pages 174-193, ISSN 2468-4511, https://doi.org/10.1016/j.cobme.2017.10.011. (http://www.sciencedirect.com/science/article/pii/S2468451117300636) | |||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
ID Code: | 83200 | |||||||||
Collection: | CaltechAUTHORS | |||||||||
Deposited By: | Tony Diaz | |||||||||
Deposited On: | 14 Nov 2017 23:12 | |||||||||
Last Modified: | 15 Nov 2021 19:56 |
Repository Staff Only: item control page