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RAP-MS: A Method to Identify Proteins that Interact Directly with a Specific RNA Molecule in Cells

McHugh, Colleen A. and Guttman, Mitchell (2017) RAP-MS: A Method to Identify Proteins that Interact Directly with a Specific RNA Molecule in Cells. In: RNA Detection: Methods and Protocols. Methods in Molecular Biology. No.1649. Humana Press , New York, NY, pp. 473-488. ISBN 978-1-4939-7212-8. https://resolver.caltech.edu/CaltechAUTHORS:20171115-111045657

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Abstract

RNA molecules interact with proteins to perform a variety of functions in living cells. The binding partners of many RNAs, in particular the newly discovered class of long noncoding RNAs (lncRNAs), remain largely unknown. RNA antisense purification coupled with mass spectrometry (RAP-MS) is a method that enables the identification of direct and specific protein interaction partners of a specific RNA molecule. Because RAP-MS uses direct RNA–protein cross-linking methods coupled along with highly denaturing purification conditions, RAP-MS provides a short list of high confidence protein interactors.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1007/978-1-4939-7213-5_31DOIArticle
https://link.springer.com/protocol/10.1007%2F978-1-4939-7213-5_31PublisherArticle
ORCID:
AuthorORCID
Guttman, Mitchell0000-0003-4748-9352
Additional Information:© 2018 Springer Science+Business Media LLC. First Online: 13 November 2017.
Subject Keywords:RNA–protein interactions; UV cross-linking; Mass spectrometry; RNA purification; Protein purification; Antisense nucleic acid capture; RNA-binding proteins
Series Name:Methods in Molecular Biology
Issue or Number:1649
Record Number:CaltechAUTHORS:20171115-111045657
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20171115-111045657
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:83227
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:15 Nov 2017 21:48
Last Modified:03 Oct 2019 19:03

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