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A rapid-response ultrasensitive biosensor for influenza virus detection using antibody modified boron-doped diamond

Nidzworski, Dawid and Siuzdak, Katarzyna and Niedziałkowski, Paweł and Bogdanowicz, Robert and Sobaszek, Michał and Ryl, Jacek and Weiher, Paulina and Sawczak, Mirosław and Wnuk, Elżbieta and Goddard, William A., III and Jaramillo-Botero, Andres and Ossowski, Tadeusz (2017) A rapid-response ultrasensitive biosensor for influenza virus detection using antibody modified boron-doped diamond. Scientific Reports, 7 (1). Art. No. 15707. ISSN 2045-2322. PMCID PMC5691202. https://resolver.caltech.edu/CaltechAUTHORS:20171117-081409225

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Abstract

According to the World Health Organization (WHO), almost 2 billion people each year are infected worldwide with flu-like pathogens including influenza. This is a contagious disease caused by viruses belonging to the family Orthomyxoviridae. Employee absenteeism caused by flu infection costs hundreds of millions of dollars every year. To successfully treat influenza virus infections, detection of the virus during the initial development phase of the infection is critical, when tens to hundreds of virus-associated molecules are present in the patient’s pharynx. In this study, we describe a novel universal diamond biosensor, which enables the specific detection of the virus at ultralow concentrations, even before any clinical symptoms arise. A diamond electrode is surface-functionalized with polyclonal anti-M1 antibodies, which then serve to identify the universal biomarker for the influenza virus, M1 protein. The absorption of the M1 protein onto anti-M1 sites of the electrode change its electrochemical impedance spectra. We achieved a limit of detection of 1 fg/ml in saliva buffer for the M1 biomarker, which corresponds to 5–10 viruses per sample in 5 minutes. Furthermore, the universality of the assay was confirmed by analyzing different strains of influenza A virus.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1038/s41598-017-15806-7DOIArticle
https://www.nature.com/articles/s41598-017-15806-7PublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5691202/PubMed CentralArticle
ORCID:
AuthorORCID
Bogdanowicz, Robert0000-0002-7543-2620
Goddard, William A., III0000-0003-0097-5716
Jaramillo-Botero, Andres0000-0003-2844-0756
Additional Information:© 2017 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. Received: 03 April 2017; Accepted: 02 November 2017; Published online: 16 November 2017. This work was supported by the Polish National Science Center (NCN) under Grant No. 2014/14/M/ST5/00715, 2015/17/D/ST5/02571 and 2016/21/B/ST7/01430. Next, we would like to acknowledge the Foundation for Polish Science (FNP) and Mazovian EU Programme grant no. RPMA.01.02.00-14-6231/16-00. The DS funds of the Faculty of Electronics, Telecommunications, and Informatics and the Faculty of Chemistry at the Gdansk University of Technology are also acknowledged. R. Bogdanowicz wants to thank Prof. W. A. Goddard for the invitation and hosting in California Institute of Technology. The Fulbright Commission is acknowledged for financial support of this fellowship. Author Contributions: D.N., R.B., T.O. and A.J.B. conceived and designed the work. D.N. and P.W. produced the recombinant protein and polyclonal antibody, R.B. and M.So. prepared the electrode, P.N. and T.O. modified electrodes, K.S., M.S., and E.W. optimized the assay and performed analysis, D.N., K.S., P.N., and R.B. wrote the manuscript with corrections from all authors. Theoretical and computational validation models where conceived and prepared by W.A.G., A.J.B., and R.B. J.R. was responsible for XPS studies. All the authors analyzed the data, discussed the results, and reviewed the manuscript. The authors declare that they have no competing interests.
Funders:
Funding AgencyGrant Number
National Science Centre (Poland)2014/14/M/ST5/00715
National Science Centre (Poland)2015/17/D/ST5/02571
National Science Centre (Poland)2016/21/B/ST7/01430
Foundation for Polish ScienceUNSPECIFIED
Mazovian EU ProgrammeRPMA.01.02.00-14-6231/16-00
Gdansk University of TechnologyUNSPECIFIED
Fulbright CommissionUNSPECIFIED
Other Numbering System:
Other Numbering System NameOther Numbering System ID
WAG1244
Issue or Number:1
PubMed Central ID:PMC5691202
Record Number:CaltechAUTHORS:20171117-081409225
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20171117-081409225
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:83280
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:20 Nov 2017 18:58
Last Modified:03 Oct 2019 19:04

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