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Enzyme Nicotinamide Cofactor Specificity Reversal Guided by Automated Structural Analysis and Library Design

Cahn, Jackson K. B. and Brinkmann-Chen, Sabine and Arnold, Frances H. (2017) Enzyme Nicotinamide Cofactor Specificity Reversal Guided by Automated Structural Analysis and Library Design. In: Synthetic Metabolic Pathways. Methods in Molecular Biology. No.1671. Humana Press , New York, NY, pp. 15-26. ISBN 978-1-4939-7294-4. https://resolver.caltech.edu/CaltechAUTHORS:20171127-143418550

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Abstract

The specificity of enzymes for nicotinamide adenine dinucleotide (NAD) or nicotinamide adenine dinucleotide phosphate (NADP) as redox carriers can pose a significant hurdle for metabolic engineering and synthetic biology applications, where switching the specificity might be beneficial. We have developed an easy-to-use computational tool (CSR-SALAD) for the design of mutant libraries to simplify the process of reversing the cofactor specificity of an enzyme. Here, we describe the optimal use of this tool and present methods for its application in a laboratory setting.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1007/978-1-4939-7295-1_2DOIArticle
https://link.springer.com/protocol/10.1007%2F978-1-4939-7295-1_2PublisherArticle
ORCID:
AuthorORCID
Brinkmann-Chen, Sabine0000-0002-5419-4192
Arnold, Frances H.0000-0002-4027-364X
Additional Information:© 2018 Springer Science+Business Media, LLC.
Subject Keywords:Cofactor specificity; Cofactor switch; Protein engineering; Library design; Oxidoreductases
Series Name:Methods in Molecular Biology
Issue or Number:1671
Record Number:CaltechAUTHORS:20171127-143418550
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20171127-143418550
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:83459
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:27 Nov 2017 22:40
Last Modified:14 Oct 2019 18:02

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