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Study of Copper Accumulation by the Type I Methanotroph Methylomicrobium albus BG8

Berson, Olga and Lidstrom, Mary E. (1996) Study of Copper Accumulation by the Type I Methanotroph Methylomicrobium albus BG8. Environmental Science and Technology, 30 (3). pp. 802-809. ISSN 0013-936X. https://resolver.caltech.edu/CaltechAUTHORS:20171129-160848761

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Abstract

Copper accumulation, which includes both sorption and uptake, has been investigated in the type I methanotroph Methylomicrobium albus BG8 at copper concentrations that were neither growth limiting nor toxic. The disappearance of the cupric ion [Cu^(2+)(H_2O)_6] from the growth medium was studied using a copper-selective electrode and was compared with total copper accumulation by the cells assayed by inductively coupled plasma/mass spectrometry. The amount of copper accumulated by the cells was related to the cupric ion concentration rather than to that of the total copper added to the growth medium. Copper accumulation demonstrated a hyperbolic dependence on cupric ion concentration, suggesting the saturation of copper binding sites on the cell surface. A mean maximum binding capacity was estimated as 1.54 × 10^(-15) mol/cell and an apparent half-saturation constant as 1.43 × 10^(-7) mol/L. Sorption experiments established that most of the copper accumulated by the cells was removable by EDTA. The portion of the copper in the cellular fraction that was not removed by EDTA showed a rapid saturation at 1−3 × 10^(-17) mol of copper/cell despite a 100-fold variation in medium total copper and cupric ion concentrations. The copper accumulated by the cells that was EDTA-removable was not available for either direct or indirect uptake by the cells.


Item Type:Article
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URLURL TypeDescription
https://doi.org/10.1021/es950212+DOIArticle
http://pubs.acs.org/doi/abs/10.1021/es950212%2BPublisherArticle
Additional Information:© 1996 American Chemical Society. Received for review March 28, 1995. Revised manuscript received September 18, 1995. Accepted October 16, 1995. We thank Prof. J. J. Morgan and Prof. S. I. Chan for their helpful comments. This work was supported by grants from the Mellon Foundation, the Office of Naval Research (N00014-91-J-1899), and the ARPA (N00014-92-J-1901).
Funders:
Funding AgencyGrant Number
Andrew W. Mellon FoundationUNSPECIFIED
Office of Naval Research (ONR)N00014-91-J-1899
Advanced Research Projects Agency (ARPA)N00014-92-J-1901
Issue or Number:3
Record Number:CaltechAUTHORS:20171129-160848761
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20171129-160848761
Official Citation:Study of Copper Accumulation by the Type I Methanotroph Methylomicrobium albus BG8. Olga Berson and Mary E. Lidstrom. Environmental Science & Technology 1996 30 (3), 802-809. DOI: 10.1021/es950212+
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:83579
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:30 Nov 2017 00:15
Last Modified:03 Oct 2019 19:08

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