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Sustained Thromboresistant Bioactivity with Reduced Intimal Hyperplasia of Heparin-Bonded PTFE Propaten Graft in a Chronic Canine Femoral Artery Bypass Model

Freeman, John and Chen, Aaron and Weinberg, Roy J. and Okada, Tamuru and Chen, Changyi and Lin, Peter H. (2018) Sustained Thromboresistant Bioactivity with Reduced Intimal Hyperplasia of Heparin-Bonded PTFE Propaten Graft in a Chronic Canine Femoral Artery Bypass Model. Annals of Vascular Surgery, 49 . pp. 295-303. ISSN 0890-5096. https://resolver.caltech.edu/CaltechAUTHORS:20171211-154548409

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Abstract

Background: Bypass graft thrombosis remains a significant mode of failure in prosthetic graft revascularization. The purpose of this investigation was to evaluate the long-term thromboresistant effect of heparin-bonded expanded polytetrafluoroethylene (ePTFE) graft using Carmeda BioActive Surface technology in a canine model. Methods: Bilateral femorofemoral artery bypass grafts with ePTFE grafts were performed in 25 adult grayhound dogs. In each animal, a heparin-bonded ePTFE graft (Propaten, WL Gore) was placed on one side, whereas a control nonheparin graft was placed on the contralateral side. The graft patency was assessed at 1, 6, 12, 18, and 24 months (n = 5 per group) following the bypass. Heparin bioactivity of the graft material was analyzed. The effect of intimal hyperplasia was also assessed. Results: All bypass grafts were patent at 1 month. Significantly greater patency rates were noted in the Propaten group compared to the control group at 12, 18, and 24 months, which were 84%, 80%, and 80% vs. 55%, 35%, and 20%, respectively (P < 0.02). There was a significant reduction in the anastomotic neointimal area and neointimal cell proliferation in Propaten grafts compared with control grafts at all groups between 6 and 24 months (P < 0.05). Heparin bioactivity as measured by antithrombin binding assay was demonstrated in the Propaten graft between 1 and 24 months. Mean heparin activities on Propaten grafts ranged from 26.3 ± 6.4 pmol/cm2 to 18.4 ± 8.7 pmol/cm2 between 1 and 24 months, which were significantly greater than the control group (P < 0.001). Differences between mean heparin activities of explanted Propaten graft samples at the various time points were nonsignificant (P > 0.05). Conclusions: Heparin-bonded ePTFE graft provides a thromboresistant surface and reduced anastomotic intimal hyperplasia at 2 years. The stable heparin bioactivity of the Propaten graft confers an advantage in long-term graft patency.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.avsg.2017.09.017DOIArticle
http://www.sciencedirect.com/science/article/pii/S0890509617310981PublisherArticle
Additional Information:© 2017 The Author(s). Published by Elsevier Inc. Under a Creative Commons license (CC BY-NC-ND 4.0). Received 8 May 2017, Revised 27 August 2017, Accepted 20 September 2017, Available online 29 November 2017. Presented at the 35th Annual Meeting of the Southern California Vascular Surgery Society, Rancho Mirage, CA, May 5-7, 2017.
Subject Keywords:heparin bonded PTFE graft; thromboresistant bioactivity; canine model
Record Number:CaltechAUTHORS:20171211-154548409
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20171211-154548409
Official Citation:John Freeman, Aaron Chen, Roy J. Weinberg, Tamuru Okada, Changyi Chen, Peter H. Lin, Sustained Thromboresistant Bioactivity with Reduced Intimal Hyperplasia of Heparin-Bonded Polytetrafluoroethylene Propaten Graft in a Chronic Canine Femoral Artery Bypass Model, Annals of Vascular Surgery, Volume 49, 2018, Pages 295-303, ISSN 0890-5096, https://doi.org/10.1016/j.avsg.2017.09.017. (http://www.sciencedirect.com/science/article/pii/S0890509617310981)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:83815
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:12 Dec 2017 21:08
Last Modified:03 Oct 2019 19:11

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