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Aerobic and Anaerobic Methanotrophic Communities Associated with Methane Hydrates Exposed on the Seafloor: A High-Pressure Sampling and Stable Isotope-Incubation Experiment

Case, David H. and Ijiri, Akira and Morono, Yuki and Tavormina, Patricia and Orphan, Victoria J. and Inagaki, Fumio (2017) Aerobic and Anaerobic Methanotrophic Communities Associated with Methane Hydrates Exposed on the Seafloor: A High-Pressure Sampling and Stable Isotope-Incubation Experiment. Frontiers in Microbiology, 8 . Art. No. 2569. ISSN 1664-302X. http://resolver.caltech.edu/CaltechAUTHORS:20180105-082606163

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Abstract

High-pressure (HP) environments represent the largest volumetric majority of habitable space for microorganisms on the planet, including the deep-sea and subsurface biosphere. However, the importance of pressure as an environmental variable affecting deep microbial life and their biogeochemical functions in carbon cycling still remains poorly understood. Here, we designed a new high-volume HP-sediment core sampler that is deployable on the payload of a remotely operated vehicle and can maintain in situ HP conditions throughout multi-month enrichment incubations including daily amendments with liquid media and gases and daily effluent sampling for geochemical or microbiological analysis. Using the HP core device, we incubated sediment and overlying water associated with methane hydrate-exposed on the seafloor of the Joetsu Knoll, Japan, at 10 MPa and 4°C for 45 days in the laboratory. Diversity analyses based on 16S rRNA and methane-related functional genes, as well as carbon isotopic analysis of methane and bicarbonate, indicated the stimulation of both aerobic and anaerobic methanotrophy driven by members of the Methylococcales, and ANME, respectively: i.e., aerobic methanotrophy was observed upon addition of oxygen whereas anaerobic processes subsequently occurred after oxygen consumption. These laboratory-measured rates at 10 MPa were generally in agreement with previously reported rates of methane oxidation in other oceanographic locations.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.3389/fmicb.2017.02569DOIArticle
https://www.frontiersin.org/articles/10.3389/fmicb.2017.02569/fullPublisherArticle
ORCID:
AuthorORCID
Orphan, Victoria J.0000-0002-5374-6178
Additional Information:© 2017 Case, Ijiri, Morono, Tavormina, Orphan and Inagaki. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Received: 15 August 2017; Accepted: 11 December 2017; Published: 19 December 2017. Author Contributions: FI and VO: designed the research; FI: led the NT13–15 cruise; YM and FI: designed the high-pressure sampling and incubation system; DC, AI, YM, and FI: collected the deep-sea samples; DC, AI, and YM: performed a high-pressure incubation experiment; AI: performed geochemical analyses; DC, PT, and VO performed molecular analyses; DC and AI: wrote the manuscript with significant input from FI and VO; All authors contributed to interpretation of data. This study was supported in part by the Japan Society for the Promotion of Science (JSPS) Strategic Fund for Strengthening Leading-Edge Research and Development (to JAMSTEC and FI), the JSPS Funding Program for Next Generation World-Leading Researchers (GR102 to FI), JSPS Grant-in-Aid for Scientific Research (24687004 to YM and 26251041 to FI), DC was supported by a National Science Foundation (NSF) Graduate Research Fellowship as well as the East Asian and Pacific Summer Institutes (EAPSI) Summer Fellowship, co-funded by the NSF and JSPS. The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. We thank the crew of the R/V Natsushima cruise NT13-15, for their help at sea, without which this work would not have been possible. We also thank Makoto Nagasawa and Masamitsu Matsumoto (Syn Co. Inc.) for the construction of high-pressure devices and useful advices. Masazumi Tsutsumi, Yoko Ohtomo, Go-Ichiro Uramoto, Tatsuhiko Hoshino, Takeshi Terada, Nan Xiao helped with field and laboratory work. This is a contribution to the Deep Carbon Observatory.
Funders:
Funding AgencyGrant Number
Japan Society for the Promotion of Science (JSPS)GR102
Japan Society for the Promotion of Science (JSPS)24687004
Japan Society for the Promotion of Science (JSPS)26251041
NSF Graduate Research FellowshipUNSPECIFIED
East Asian and Pacific Summer Institutes (EAPSI) Summer FellowshipUNSPECIFIED
Subject Keywords:methanotrophs, high pressure incubation, methane hydrate, stable isotope probing, marine sediment
Record Number:CaltechAUTHORS:20180105-082606163
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20180105-082606163
Official Citation:Case DH, Ijiri A, Morono Y, Tavormina P, Orphan VJ and Inagaki F (2017) Aerobic and Anaerobic Methanotrophic Communities Associated with Methane Hydrates Exposed on the Seafloor: A High-Pressure Sampling and Stable Isotope-Incubation Experiment. Front. Microbiol. 8:2569. doi: 10.3389/fmicb.2017.02569
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:84116
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:05 Jan 2018 18:26
Last Modified:05 Jan 2018 18:26

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