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Morphological analysis of the axonal projections of EGFP-labeled Esr1-expressing neurons in transgenic female medaka

Zempo, Buntaro and Karigo, Tomomi and Kanda, Shinji and Akazome, Yasuhisa and Oka, Yoshitaka (2018) Morphological analysis of the axonal projections of EGFP-labeled Esr1-expressing neurons in transgenic female medaka. Endocrinology, 159 (2). pp. 1228-1241. ISSN 0013-7227. http://resolver.caltech.edu/CaltechAUTHORS:20180108-132446706

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Abstract

Some hypothalamic neurons expressing estrogen receptor α (Esr1) are thought to transmit a gonadal estrogen feedback signal to gonadotropin releasing hormone 1 (GnRH1) neurons, which is the final common pathway for feedback regulation of reproductive functions. Moreover, estrogen-sensitive neurons are suggested to control sexual behaviors in coordination with reproduction. In mammals, hypothalamic estrogen-sensitive neurons release the peptide Kisspeptin and regulate GnRH1 neurons. However, a growing body of evidence in non-mammalian species casts doubt on the regulation of GnRH1 neurons by Kisspeptin neurons. As a first step towards understanding how estrogen regulates neuronal circuits for reproduction and sex behavior in vertebrates in general, we generated a transgenic medaka that expresses EGFP specifically in Esr1-expressing neurons (Esr1 neurons) and analyzed their axonal projections. We found that Esr1 neurons in the POA project to the GnRH1 neurons. We also demonstrated, by transcriptome and histological analyses, that these Esr1 neurons are glutamatergic and/or GABAergic, but not Kisspeptinergic. We therefore suggest that glutamatergic/GABAergic Esr1 neurons in the POA regulate GnRH1 neurons. This hypothesis is consistent with previous studies in mice that glutamatergic/GABAergic transmission is critical for estrogen-dependent changes in GnRH1 neuron firing. Thus, we propose that this neuronal circuit may provide an evolutionarily conserved mechanism for regulation of reproduction. In addition, we showed that telencephalic Esr1 neurons project to medulla, which may control sexual behavior. Moreover, we found that some POA-Esr1 neurons co-express progesterone receptors (PRs). These neurons may form the neuronal circuits that regulate reproduction and sex behavior in response to the serum estrogen/progesterone.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1210/en.2017-00873DOIArticle
https://academic.oup.com/endo/advance-article/doi/10.1210/en.2017-00873/4780796PublisherArticle
Additional Information:© 2017 Endocrine Society. Submitted: September 27, 2017; Accepted: December 20, 2017; First Online: December 28, 2017. YO Japan Society for the Promotion of Science Grant 26221104. BZ Japan Society for the Promotion of Science Grant 13J10475. The authors have nothing to disclose.
Funders:
Funding AgencyGrant Number
Japan Society for the Promotion of Science (JSPS)26221104
Japan Society for the Promotion of Science (JSPS)13J10475
Record Number:CaltechAUTHORS:20180108-132446706
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20180108-132446706
Official Citation:Buntaro Zempo, Tomomi Karigo, Shinji Kanda, Yasuhisa Akazome, Yoshitaka Oka; Morphological Analysis of the Axonal Projections of EGFP-Labeled Esr1-Expressing Neurons in Transgenic Female Medaka, Endocrinology, Volume 159, Issue 2, 1 February 2018, Pages 1228–1241, https://doi.org/10.1210/en.2017-00873
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:84168
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:08 Jan 2018 21:43
Last Modified:02 Feb 2018 23:44

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