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Site-specific collapse dynamics guide the formation of the cytochrome c' four-helix bundle

Kimura, Tetsunari and Lee, Jennifer C. and Gray, Harry B. and Winkler, Jay R. (2007) Site-specific collapse dynamics guide the formation of the cytochrome c' four-helix bundle. Proceedings of the National Academy of Sciences of the United States of America, 104 (1). pp. 117-122. ISSN 0027-8424. PMCID PMC1765420. https://resolver.caltech.edu/CaltechAUTHORS:KIMpnas07

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Abstract

The evolution of tryptophan-to-heme (W/heme) distance distributions extracted from analysis of fluorescence energy transfer kinetics during the refolding of Rhodopseudomonas palustris cytochrome c' reveals dramatic differences between two variants [W32 (Q1A/F32W/W72F) and W72 (Q1A)]. Both W32/heme and W72/heme distance distributions measured at the earliest time point attainable with a continuous-flow mixer (150 µs) confirm that the polypeptide ensemble is not uniformly collapsed and that native structure is not formed. Time-resolved fluorescence spectra indicate that W32 is sequestered from the aqueous solution during the first 700 µs of folding, whereas W72 remains exposed to solvent. The first moment of the W32/heme distance distribution evolves to its native value faster than that of W72, suggesting that the approach of W32 to the heme precedes that of W72.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1765420/PubMed CentralArticle
https://doi.org/10.1073/pnas.0609413103DOIUNSPECIFIED
https://doi.org/10.1073/pnas.0609413103DOIUNSPECIFIED
ORCID:
AuthorORCID
Gray, Harry B.0000-0002-7937-7876
Winkler, Jay R.0000-0002-4453-9716
Additional Information:© 2006 The National Academy of Sciences of the USA. Contributed by Harry B. Gray, October 24, 2006 (received for review October 12, 2006). Published online before print December 19, 2006, 10.1073/pnas.0609413103 We thank Dr. Linda Thöny-Meyer (Eidgenössiche Technische Hochschule, Zürich, Switzerland) for the ccm plasmid pEC86 and Ekaterina V. Pletneva for numerous discussions. This work was supported by National Institutes of Health Grant GM068461 (to J.R.W.), the Arnold and Mabel Beckman Foundation (Beckman Senior Research Fellowship to J.C.L.), and the Ellison Medical Foundation (Senior Scholar Award in Aging to H.B.G.). T.K. was supported by a Research Fellowship of the Japan Society for the Promotion of Science for Young Scientists. Author contributions: T.K. and J.R.W. designed research; T.K. and J.C.L. performed research; T.K. and J.C.L. analyzed data; and T.K., J.C.L., H.B.G., and J.R.W. wrote the paper. The authors declare no conflict of interest. This article contains supporting information online at www.pnas.org/cgi/content/full/0609413103/DC1.
Funders:
Funding AgencyGrant Number
NIHGM068461
Arnold and Mabel Beckman FoundationUNSPECIFIED
Ellison Medical FoundationUNSPECIFIED
Japan Society for the Promotion of Science (JSPS)UNSPECIFIED
Subject Keywords:fluorescence energy transfer, protein folding, ultrafast mixer
Issue or Number:1
PubMed Central ID:PMC1765420
Record Number:CaltechAUTHORS:KIMpnas07
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:KIMpnas07
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:8434
Collection:CaltechAUTHORS
Deposited By: Lindsay Cleary
Deposited On:13 Aug 2007
Last Modified:22 Nov 2019 09:58

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