A Caltech Library Service

The structural basis for specific decoding of AUA by isoleucine tRNA on the ribosome

Voorhees, Rebecca M. and Mandal, Debabrata and Neubauer, Cajetan and Köhrer, Caroline and RajBhandary, Uttam L. and Ramakrishnan, V. (2013) The structural basis for specific decoding of AUA by isoleucine tRNA on the ribosome. Nature Structural and Molecular Biology, 20 (5). pp. 641-643. ISSN 1545-9993. PMCID PMC3672977.

[img] PDF - Accepted Version
See Usage Policy.

[img] PDF (Supplementary Table 1) - Supplemental Material
See Usage Policy.


Use this Persistent URL to link to this item:


Decoding of the AUA isoleucine codon in bacteria and archaea requires modification of a C in the anticodon wobble position of the isoleucine tRNA. Here, we report the crystal structure of the archaeal tRNA_2^(Ile), which contains the modification agmatidine in its anticodon, in complex with the AUA codon on the 70S ribosome. The structure illustrates how agmatidine confers codon specificity for AUA over AUG.

Item Type:Article
Related URLs:
URLURL TypeDescription ReadCube access CentralArticle
Voorhees, Rebecca M.0000-0003-1640-2293
Neubauer, Cajetan0000-0002-5348-5609
Additional Information:© 2013 Macmillan Publishers Limited, part of Springer Nature. Received: 14 December 2012; Accepted: 15 February 2013; Published online: 31 March 2013. We thank A. McCarthy and S. Brockhauser at European Synchrotron Radiation Facility ID14.4 for facilitating data collection. This work was supported by the Medical Research Council, UK, grant U105184332 (V.R.), the Wellcome Trust (V.R.), the Agouron Institute (V.R.) and the Louis-Jeantet Foundation (V.R.), and grant GM17151 from the US National Institutes of Health (U.L.R.). Support was also received from the Gates-Cambridge scholarship and Peterhouse (R.V.) and from Boehringer Ingelheim (C.N.). Accession codes: Coordinates and structural factors have been deposited in the Protein Data Bank, with codes 3ZN7, 3ZN9, 3ZND and 3ZNE. Author Contributions:D.M. and C.K. purified tRNA substrates; R.M.V. and C.N. grew crystals, collected data, built and refined the structure, and analyzed the model; R.M.V., C.K., U.L.R. and V.R. wrote and edited the manuscript. The authors declare no competing financial interests.
Funding AgencyGrant Number
Medical Research Council (UK)U105184332
Agouron InstituteUNSPECIFIED
Louis-Jeantet FoundationUNSPECIFIED
Gates-Cambridge ScholarshipUNSPECIFIED
Boehringer IngelheimUNSPECIFIED
Issue or Number:5
PubMed Central ID:PMC3672977
Record Number:CaltechAUTHORS:20180116-155912862
Persistent URL:
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:84349
Deposited By: Tony Diaz
Deposited On:17 Jan 2018 17:46
Last Modified:31 Mar 2020 18:14

Repository Staff Only: item control page