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Insights into Translational Termination from the Structure of RF2 Bound to the Ribosome

Weixlbaumer, Albert and Jin, Hong and Neubauer, Cajetan and Voorhees, Rebecca M. and Petry, Sabine and Kelley, Ann C. and Ramakrishnan, Venki (2008) Insights into Translational Termination from the Structure of RF2 Bound to the Ribosome. Science, 322 (5903). pp. 953-956. ISSN 0036-8075. PMCID PMC2642913. https://resolver.caltech.edu/CaltechAUTHORS:20180117-080027343

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Abstract

The termination of protein synthesis occurs through the specific recognition of a stop codon in the A site of the ribosome by a release factor (RF), which then catalyzes the hydrolysis of the nascent protein chain from the P-site transfer RNA. Here we present, at a resolution of 3.5 angstroms, the crystal structure of RF2 in complex with its cognate UGA stop codon in the 70S ribosome. The structure provides insight into how RF2 specifically recognizes the stop codon; it also suggests a model for the role of a universally conserved GGQ motif in the catalysis of peptide release.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1126/science.1164840DOIArticle
http://science.sciencemag.org/content/sci/suppl/2008/11/06/322.5903.953.DC1/Weixlbaumer-SOM.pdfPublisherSupporting Information
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2642913PubMed CentralArticle
ORCID:
AuthorORCID
Neubauer, Cajetan0000-0002-5348-5609
Voorhees, Rebecca M.0000-0003-1640-2293
Additional Information:© 2010 American Association for the Advancement of Science. 19 August 2008; accepted 26 September 2008. We thank M. Schmeing for his useful comments and M. Fuchs and C. Schulze-Briese for their help and advice with data collection at the Swiss Light Source. This work was supported by MRC UK, a program grant from the Wellcome Trust, and awards from the Agouron Institute and Louis-Jeantet Foundation. C.N. is supported by a Boehringer-Ingelheim fellowship and R.M.V. is supported by a Gates-Cambridge fellowship. Coordinates for the structure have been deposited in the Protein Data Bank with accession codes 2jl5 to 2jl8. V.R. holds stock options in Rib-X Pharmaceuticals, a company that develops antibacterial drugs that target the ribosome.
Funders:
Funding AgencyGrant Number
Medical Research Council (UK)UNSPECIFIED
Wellcome TrustUNSPECIFIED
Agouron InstituteUNSPECIFIED
Louis-Jeantet FoundationUNSPECIFIED
Boehringer-IngelheimUNSPECIFIED
Gates-Cambridge ScholarshipUNSPECIFIED
Issue or Number:5903
PubMed Central ID:PMC2642913
Record Number:CaltechAUTHORS:20180117-080027343
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180117-080027343
Official Citation:Insights into Translational Termination from the Structure of RF2 Bound to the Ribosome BY ALBERT WEIXLBAUMER, HONG JIN, CAJETAN NEUBAUER, REBECCA M. VOORHEES, SABINE PETRY, ANN C. KELLEY, VENKI RAMAKRISHNAN Science 07 Nov 2008: Vol. 322, Issue 5903, pp. 953-956 DOI: 10.1126/science.1164840
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:84353
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:17 Jan 2018 19:07
Last Modified:31 Mar 2020 18:17

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