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DNA Damage and Apoptosis Induction in Cancer Cells by Chemically Engineered Thiolated Riboflavin Gold Nanoassembly

Sau, Abhishek and Sanyal, Sulagna and Bera, Kallol and Sen, Sabyasachi and Mitra, Amrit Krishna and Pal, Uttam and Chakraborty, Prabal Kumar and Ganguly, Sayantan and Satpati, Biswarup and Das, Chandrima and Basu, Samita (2018) DNA Damage and Apoptosis Induction in Cancer Cells by Chemically Engineered Thiolated Riboflavin Gold Nanoassembly. ACS Applied Materials & Interfaces, 10 (5). pp. 4582-4589. ISSN 1944-8244. doi:10.1021/acsami.7b18837.

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Herein we have engineered a smart nuclear targeting thiol-modified riboflavin-gold nano assembly, RfS@AuNPs, which accumulates selectively in nucleus without any nuclear-targeting peptides (NLS/RGD) and shows photophysically in vitro DNA intercalation. A theoretical model using Molecular Dynamics has been developed to probe the mechanism of formation and stability as well as dynamics of the RfS@AuNPs in aqueous solution and within DNA microenvironment. The RfS@AuNPs facilitate the binucleated cell formation that is reflected in the significant increase of DNA damage marker, γ-H2AX as well as the arrest of most of the HeLa cells at pre-G1 phase indicating cell death. Moreover, a significant upregulation of apoptotic markers confirms that the cell death occurs through apoptotic pathway. Analyses of the microarray gene expression of RfS@AuNPs treated HeLa cells show significant alterations in vital biological processes necessary for cell survival. Taken together, our study reports a unique nuclear targeting mechanism through targeting the riboflavin receptors, which are upregulated in cancer cells and induce apoptosis in the targeted cells.

Item Type:Article
Related URLs:
URLURL TypeDescription Information
Pal, Uttam0000-0003-2110-4610
Satpati, Biswarup0000-0003-1175-7562
Basu, Samita0000-0003-1629-2979
Additional Information:© 2018 American Chemical Society. Received: December 11, 2017; Accepted: January 16, 2018; Published: January 16, 2018. We sincerely thank S. Haldar, R. Modok, Y. Sikdar, C. Sengupta, P. Mitra, S. Das Chakraborty, and M. Bhattacharyya for their constant support and help. We acknowledge financial support from UGC (A.S.); F2-32/1998 (SA-1), DBT- Government of India, Ramalingaswami fellowship and BARD project: DAE at Saha Institute of Nuclear Physics. The authors declare no competing financial interest.
Funding AgencyGrant Number
University Grants Commission (India)F2-32/1998
Department of Biotechnology (India)UNSPECIFIED
Department of Atomic Energy (India)UNSPECIFIED
Subject Keywords:riboflavin gold nanoassembly, nuclear targeting, DNA damage response, apoptotic cell death, global gene expression
Issue or Number:5
Record Number:CaltechAUTHORS:20180117-092127661
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Official Citation:DNA Damage and Apoptosis Induction in Cancer Cells by Chemically Engineered Thiolated Riboflavin Gold Nanoassembly. Abhishek Sau, Sulagna Sanyal, Kallol Bera, Sabyasachi Sen, Amrit Krishna Mitra, Uttam Pal, Prabal Kumar Chakraborty, Sayantan Ganguly, Biswarup Satpati, Chandrima Das, and Samita Basu. ACS Applied Materials & Interfaces 2018 10 (5), 4582-4589 DOI: 10.1021/acsami.7b18837
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:84358
Deposited By: Tony Diaz
Deposited On:17 Jan 2018 19:11
Last Modified:15 Nov 2021 20:19

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