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Evidence for amino acid sequence differences among proteins resembling the L-chain subunits of immunoglobulins

Bennett, J. Claude and Hood, Leroy E. and Dreyer, William J. and Potter, Michael (1965) Evidence for amino acid sequence differences among proteins resembling the L-chain subunits of immunoglobulins. Journal of Molecular Biology, 12 (1). pp. 81-87. ISSN 0022-2836. doi:10.1016/S0022-2836(65)80284-4.

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In this study immunoglobulin structural variation was examined through analysis of L-chain type proteins produced by a variety of distinct plasma cell tumors of the mouse. These proteins were examined by serological techniques, quantitative amino acid analysis and peptide mapping. It appeared from these studies that these proteins all contained a common amino acid sequence throughout a large portion of the molecule. Nevertheless, each of the proteins contained a unique region of amino acid sequence which allowed it to be distinguished from any other on the basis of certain variable peptides. This immediately suggested the possibility of a second chain, loosely linked by non-covalent bonding or joined through disulfide bonding. To examine this possibility, performic acid oxidation and disulfide reduction procedures were carried out. Following these reactions, several methods of separation failed to demonstrate any alteration in either the peptide map patterns or the amino acid composition of these proteins. It seems, therefore, that if a second chain is present it is covalently linked through some bond other than a disulfide bond. It is apparent from the peptide map data that variation must occur in some type of “specialized area”. Although the role of the various chains of immune globulins is not yet clear, we believe that this remarkable type of amino acid sequence variation reflects in part the mechanism by which specificity is conferred.

Item Type:Article
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Hood, Leroy E.0000-0001-7158-3678
Additional Information:© 1965 Published by Elsevier Ltd. Received 29 December 1964. We thank Mr John Raes for his excellent technical assistance during the course of this work, which was supported in part by a Public Health Service Research Grant (GMO-6965) and by a Public Health Postdoctoral Fellowship (I-F2-GM-20, 388-01) (LH).
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NIH Postdoctoral FellowshipI-F2-GM-20, 388-01
Issue or Number:1
Record Number:CaltechAUTHORS:20180122-104213896
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Official Citation:J. Claude Bennett, Leroy E. Hood, William J. Dreyer, Michael Potter, Evidence for amino acid sequence differences among proteins resembling the L-chain subunits of immunoglobulins, Journal of Molecular Biology, Volume 12, Issue 1, 1965, Pages 81-87, ISSN 0022-2836, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:84453
Deposited By: Tony Diaz
Deposited On:23 Jan 2018 13:35
Last Modified:15 Nov 2021 20:19

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