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Enzymatic deamidation of methyl-accepting chemotaxis proteins in Escherichia coli catalyzed by the cheB gene product

Kehry, Marilyn R. and Bond, Martha W. and Hunkapiller, Michael W. and Dahlquist, Frederick W. (1983) Enzymatic deamidation of methyl-accepting chemotaxis proteins in Escherichia coli catalyzed by the cheB gene product. Proceedings of the National Academy of Sciences of the United States of America, 80 (12). pp. 3599-3603. ISSN 0027-8424.

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The methyl-accepting chemotaxis proteins (MCPs) of Escherichia coli undergo reversible methylation that has been correlated with adaptation of cells to environmental stimuli. MCPI, the product of the tsr gene, accepts methyl groups at multiple sites that are located on two tryptic peptides, denoted K1 and R1. A second modification of the MCPs, which is not methylation, has been designated the CheB-dependent modification. A CheB-dependent modification occurs on methyl-accepting peptide K1 and allows additional methyl groups to be incorporated into this peptide. We have performed partial amino acid sequence analyses on radiolabeled peptides K1 and R1 derived from MCPI and have identified several methyl-accepting sites. We found that, in the absence of CheB-dependent modification, a site in peptide K1 is unable to accept methyl groups. Correlation of this protein sequence data with the nucleotide sequence of the tsr gene [Boyd, A., Kendall, K. & Simon, M. I. (1983) Nature (London) 301, 623-626] suggests that CheB-dependent modification of MCPI is the enzymatic deamidation of glutamine to methyl-accepting glutamic acid. Possible roles for this deamidation in bacterial chemotaxis are discussed.

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Additional Information:© 1983 by the National Academy of Sciences. Communicated by George Streisinger, March 11, 1983. We thank J.S. Parkinson, M. Silverman, and M. Simon for their generous gifts of phage and bacterial strains, A. Boyd for communicating nucleotide sequence results prior to publication, L. Hood for use of the protein sequence facilities, and N. Hirata for preparation of the manuscript. This work was supported by Damon Runyon-Walter Winchell Cancer Fund Fellowship DRG-461 and National Institutes of Health Grant AI06375 to M.R.K. and National Institutes of Health Grant GM 24792 to F.W.D.; F.W.D. is the recipient of an American Cancer Society Faculty Research Award. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:methylation; CheB-dependent modification; sensory adaptation
Issue or Number:12
Record Number:CaltechAUTHORS:KEHpnas83
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:8447
Deposited By: Tony Diaz
Deposited On:14 Aug 2007
Last Modified:02 Oct 2019 23:51

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