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Molecularly Imprinted Polymer-Based Catalytic Micromotors for Selective Protein Transport

Orozco, Jahir and Cortés, Allan and Cheng, Guanzhi and Sattayasamitsathit, Sirilak and Gao, Wei and Feng, Xiaomiao and Shen, Yufeng and Wang, Joseph (2013) Molecularly Imprinted Polymer-Based Catalytic Micromotors for Selective Protein Transport. Journal of the American Chemical Society, 135 (14). pp. 5336-5339. ISSN 0002-7863. http://resolver.caltech.edu/CaltechAUTHORS:20180123-150633925

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Abstract

We demonstrate an attractive nanomachine “capture and transport” target isolation strategy based on molecularly imprinted polymers (MIPs). MIP-based catalytic microtubular engines are prepared by electropolymerization of the outer polymeric layer in the presence of the target analyte (template). Tailor-made selective artificial recognition sites are thus introduced into the tubular microtransporters through complementary nanocavities in the outer polymeric layer. The new microtransporter concept is illustrated using bilayer poly(3,4-ethylenedioxythiophene) (PEDOT)/Pt–Ni microengines and fluorescein isothiocyanate (FITC)-labeled avidin (Av-FITC) as the template. The avidin-imprinted polymeric layer selectively concentrates the fluorescent-tagged protein target onto the moving microengine without the need for additional external functionalization, allowing “on-the-fly” extraction and isolation of Av-FITC from raw serum and saliva samples along with real-time visualization of the protein loading and transport. The new micromachine–MIP-based target isolation strategy can be extended to the capture and transport of other important target molecules, leading toward diverse biomedical and environmental applications.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja4018545DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja4018545PublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/ja4018545PublisherSupporting Information
Additional Information:© 2013 American Chemical Society. Received 20 February 2013. Published online 26 March 2013. Published in print 10 April 2013. This project received support from the Defense Threat Reduction Agency–Joint Science and Technology Office for Chemical and Biological Defense (Grant HDTRA1-13-1-0002). J.O. acknowledges financial support from the Beatriu de Pinós Fellowship (Government of Catalonia). G.C. and X.F. were supported by joint-supervised Ph.D. programs with the China Scholarship Council and the Jiangsu Overseas Research & Training Program, respectively. W.G. is a Howard Hughes Medical Institute International Student Research Fellow. The authors thank G. Galicia for his assistance. The authors declare no competing financial interest.
Funders:
Funding AgencyGrant Number
Defense Threat Reduction Agency (DTRA)HDTRA1-13-1-0002
Beatriu de Pinós (Catalonia)UNSPECIFIED
China Scholarship CouncilUNSPECIFIED
Jiangsu Overseas Research & Training ProgramUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Record Number:CaltechAUTHORS:20180123-150633925
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20180123-150633925
Official Citation:Molecularly Imprinted Polymer-Based Catalytic Micromotors for Selective Protein Transport Jahir Orozco, Allan Cortés, Guanzhi Cheng, Sirilak Sattayasamitsathit, Wei Gao, Xiaomiao Feng, Yufeng Shen, and Joseph Wang Journal of the American Chemical Society 2013 135 (14), 5336-5339 DOI: 10.1021/ja4018545
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:84484
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:31 Jan 2018 22:35
Last Modified:31 Jan 2018 22:35

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