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Micromotor-based lab-on-chip immunoassays

García, Miguel and Orozco, Jahir and Guix, Maria and Gao, Wei and Sattayasamitsathit, Sirilak and Escarpa, Alberto and Merkoçi, Arben and Wang, Joseph (2013) Micromotor-based lab-on-chip immunoassays. Nanoscale, 5 (4). pp. 1325-1331. ISSN 2040-3364. http://resolver.caltech.edu/CaltechAUTHORS:20180124-084611121

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Abstract

Here we describe the first example of using self-propelled antibody-functionalized synthetic catalytic microengines for capturing and transporting target proteins between the different reservoirs of a lab-on-a-chip (LOC) device. A new catalytic polymer/Ni/Pt microtube engine, containing carboxy moieties on its mixed poly(3,4-ethylenedioxythiophene) (PEDOT)/COOH–PEDOT polymeric outermost layer, is further functionalized with the antibody receptor to selectively recognize and capture the target protein. The new motor-based microchip immunoassay operations are carried out without any bulk fluid flow, replacing the common washing steps in antibody-based protein bioassays with the active transport of the captured protein throughout the different reservoirs, where each step of the immunoassay takes place. A first microchip format involving an ‘on-the-fly’ double-antibody sandwich assay (DASA) is used for demonstrating the selective capture of the target protein, in the presence of excess of non-target proteins. A secondary antibody tagged with a polymeric-sphere tracer allows the direct visualization of the binding events. In a second approach the immuno-nanomotor captures and transports the microsphere-tagged antigen through a microchannel network. An anti-protein-A modified microengine is finally used to demonstrate the selective capture, transport and convenient label-free optical detection of a Staphylococcus aureus target bacteria (containing proteinA in its cell wall) in the presence of a large excess of non-target (Saccharomyces cerevisiae) cells. The resulting nanomotor-based microchip immunoassay offers considerable potential for diverse applications in clinical diagnostics, environmental and security monitoring fields.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1039/C2NR32400HDOIArticle
http://pubs.rsc.org/en/content/articlehtml/2013/nr/c2nr32400hPublisherArticle
Additional Information:© 2013 Royal Society of Chemistry. Received 21st August 2012 , Accepted 11th October 2012. First published on 23rd October 2012. This work was supported by the U.S. DTRA Grant HDTRA1-13-1-0002 and NATO Science for Peace and Security Program (SfP 983807). M. G. G., J.O. and M. G. acknowledge financial support from the University of Alcalá (Madrid), the Government of Catalonia and the Spanish MICINN, respectively. W.G. is a Howard Hughes Medical Institute International Student Research fellow. We also thank Allan Cortés and Ashley Pourazary for their support in the experiments.
Funders:
Funding AgencyGrant Number
Defense Threat Reduction Agency (DTRA)HDTRA1-13-1-0002
North Atlantic Treaty Organization (NATO)SfP 983807
University of AlcaláUNSPECIFIED
Beatriu de Pinós (Catalonia)UNSPECIFIED
Ministerio de Ciencia e Innovación (MCINN)UNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Record Number:CaltechAUTHORS:20180124-084611121
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20180124-084611121
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:84494
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:31 Jan 2018 22:29
Last Modified:31 Jan 2018 22:36

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