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Bacterial Isolation by Lectin-Modified Microengines

Campuzano, Susana and Orozco, Jahir and Kagan, Daniel and Guix, Maria and Gao, Wei and Sattayasamitsathit, Sirilak and Claussen, Jonathan C. and Merkoçi, Arben and Wang, Joseph (2012) Bacterial Isolation by Lectin-Modified Microengines. Nano Letters, 12 (1). pp. 396-401. ISSN 1530-6984. http://resolver.caltech.edu/CaltechAUTHORS:20180126-104905077

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Abstract

New template-based self-propelled gold/nickel/polyaniline/platinum (Au/Ni/PANI/Pt) microtubular engines, functionalized with the Concanavalin A (ConA) lectin bioreceptor, are shown to be extremely useful for the rapid, real-time isolation of Escherichia coli (E. coli) bacteria from fuel-enhanced environmental, food, and clinical samples. These multifunctional microtube engines combine the selective capture of E. coli with the uptake of polymeric drug-carrier particles to provide an attractive motion-based theranostics strategy. Triggered release of the captured bacteria is demonstrated by movement through a low-pH glycine-based dissociation solution. The smaller size of the new polymer-metal microengines offers convenient, direct, and label-free optical visualization of the captured bacteria and discrimination against nontarget cells.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/nl203717qDOIArticle
http://pubs.acs.org/doi/abs/10.1021/nl203717qPublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/nl203717qPublisherSupporting Information
Additional Information:© 2011 American Chemical Society. Received 21 October 2011. Published online 5 December 2011. Published in print 11 January 2012. This work was supported by the National Science Foundation (Award Number CBET 0853375), the National Institutes of Health (Award U01 AI075565), NATO Science for Peace and Security Program (SfP 983807), and the Laboratory Directed Research and Development program at Sandia National Laboratories. S.C. and J.O. acknowledge the support from Programa Becas Complutense del Amo (2010-2011) and a Beatriu de Pinós postdoctoral fellowship (Government of Catalonia), respectively. M.G. thanks the University Autónoma of Barcelona (UAB) and the Spanish MICINN. The authors would like to acknowledge also B. Chuluun and C. Halford for their technical assistance.
Funders:
Funding AgencyGrant Number
NSFCBET-0853375
NIHU01 AI075565
North Atlantic Treaty Organization (NATO)SfP 983807
Sandia National LaboratoriesUNSPECIFIED
Programa Becas Complutense del Amo (2010-2011)UNSPECIFIED
Beatriu de Pinós (Catalonia)UNSPECIFIED
University Autónoma of BarcelonaUNSPECIFIED
Ministerio de Ciencia e Innovación (MCINN)UNSPECIFIED
Subject Keywords:Nanomachines, microengines, lectin, E. coli isolation, theranostics, drug delivery, biodetection, complex samples
Record Number:CaltechAUTHORS:20180126-104905077
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20180126-104905077
Official Citation:Bacterial Isolation by Lectin-Modified Microengines Susana Campuzano, Jahir Orozco, Daniel Kagan, Maria Guix, Wei Gao, Sirilak Sattayasamitsathit, Jonathan C. Claussen, Arben Merkoçi, and Joseph Wang Nano Letters 2012 12 (1), 396-401 DOI: 10.1021/nl203717q
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:84543
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:31 Jan 2018 19:50
Last Modified:31 Jan 2018 19:50

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