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Dynamic Isolation and Unloading of Target Proteins by Aptamer-Modified Microtransporters

Orozco, Jahir and Campuzano, Susana and Kagan, Daniel and Zhou, Ming and Gao, Wei and Wang, Joseph (2011) Dynamic Isolation and Unloading of Target Proteins by Aptamer-Modified Microtransporters. Analytical Chemistry, 83 (20). pp. 7962-7969. ISSN 0003-2700. http://resolver.caltech.edu/CaltechAUTHORS:20180126-140204071

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Abstract

We describe here a new strategy for isolating target proteins from complex biological samples based on an aptamer-modified self-propelled microtube engine. For this purpose, a thiolated thrombin or a mixed thrombin–ATP aptamer (prehybridized with a thiolated short DNA) was coassembled with mercaptohexanol onto the gold surface of these microtube engines. The rapid movement of the aptamer-modified microtransporter resulted in highly selective and rapid capture of the target thrombin, with an effective discrimination against a large excess of nontarget proteins. Release of the captured thrombin can be triggered by the addition of ATP that can bind and displace the immobilized mixed thrombin–ATP aptamer in 20 min. The rapid loading and unloading abilities demonstrated by these selective microtransporters are illustrated in complex matrixes such as human serum and plasma. The new motion-driven protein isolation platform represents a new approach in bioanalytical chemistry based on active transport of proteins and offers considerable promise for diverse diagnostic applications.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ac202029kDOIArticle
http://pubs.acs.org/doi/abs/10.1021/ac202029kPublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/ac202029kPublisherSupporting Information
Additional Information:© 2011 American Chemical Society. Received 3 August 2011. Date accepted 2 September 2011. Published online 2 September 2011. Published in print 15 October 2011. This work was supported by the National Science Foundation (Award No. CBET 0853375). J.O. and S.C. acknowledge the support from a Beatriu de Pinós postdoctoral fellowship (Government of Catalonia) and from Programa Becas Complutense del Amo (2010–2011), respectively. The authors acknowledge B. Chuluun and N. Lim for their assistance. The first four authors have equally contributed to this work.
Funders:
Funding AgencyGrant Number
NSFCBET-0853375
Beatriu de Pinós (Catalonia)UNSPECIFIED
Programa Becas Complutense del Amo (2010–2011)UNSPECIFIED
Record Number:CaltechAUTHORS:20180126-140204071
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20180126-140204071
Official Citation:Dynamic Isolation and Unloading of Target Proteins by Aptamer-Modified Microtransporters Jahir Orozco, Susana Campuzano, Daniel Kagan, Ming Zhou, Wei Gao, and Joseph Wang Analytical Chemistry 2011 83 (20), 7962-7969 DOI: 10.1021/ac202029k
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:84547
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:31 Jan 2018 19:39
Last Modified:31 Jan 2018 19:39

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