CaltechAUTHORS
  A Caltech Library Service

Large field of view scanning fluorescence lifetime imaging system for multimode optical imaging of small animals

Hwang, Jae Youn and Agadjanian, Hasmik and Medina-Kauwe, Lali K. and Gross, Zeev and Gray, Harry B. and Sorasaenee, Karn and Farkas, Daniel L. (2008) Large field of view scanning fluorescence lifetime imaging system for multimode optical imaging of small animals. In: Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI. Proceedings of SPIE. No.6859. Society of Photo-Optical Instrumentation Engineers (SPIE) , Bellingham, WA, Art. No. 68590G. ISBN 978-0-8194-7034-8. http://resolver.caltech.edu/CaltechAUTHORS:20180205-090030536

[img] PDF - Published Version
See Usage Policy.

1615Kb

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:20180205-090030536

Abstract

We describe a scanning fluorescence lifetime imaging (SFLIM) system that provides a large field of view (LFOV), using a femtosecond (fs) pulsed laser, for multi-mode optical imaging of small animals. Fluorescence lifetime imaging (FLIM) can be a useful optical method to distinguish between fluorophores inside small animals. However, difficulty arises when LFOV is required in FLIM using a fs pulsed laser for the excitation of the fluorophores at low wavelengths (<500nm), primarily because the field of view of the pulsed blue excitation light generated from the second harmonic of the fs pulsed light is limited to about a centimeter in diameter due to the severe scattering and absorption of the light inside tissues. Here, we choose a scanning method in order to acquire a FLIM image with LFOV as one alternative. In the SFLIM system, we used a conventional cooled CCD camera coupled to an ultra-fast time-gated intensifier, a tunable femtosecond laser for the excitation of fluorophores, and an x-y moving stage for scanning. Images acquired through scanning were combined into a single image and then this reconstructed image was compared with images obtained by spectral imaging. The resulting SFLIM system is promising as an alternative method for the FLIM imaging of small animals, containing fluorophores exited by blue light, for LFOV applications such as whole animal imaging.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1117/12.769305DOIArticle
https://www.spiedigitallibrary.org/conference-proceedings-of-spie/6859/1/Large-field-of-view-scanning-fluorescence-lifetime-imaging-system-for/10.1117/12.769305.fullPublisherArticle
Additional Information:© 2008 Society of Photo-Optical Instrumentation Engineers (SPIE). We thank our colleagues, and particularly Dr. V K. Ramanujan and A. Nowatzyk for their contributions, and useful discussions. This research was supported in part by the US Navy Bureau of Medicine and Surgery.
Funders:
Funding AgencyGrant Number
US Navy Bureau of Medicine and SurgeryUNSPECIFIED
Subject Keywords:Scanning fluorescence lifetime imaging, femtosecond laser, multi-mode optical imaging, FLIM image tiling, spectral imaging, small animal imaging.
Record Number:CaltechAUTHORS:20180205-090030536
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20180205-090030536
Official Citation:Jae Youn Hwang, Hasmik Agadjanian, Lali K. Medina-Kauwe, Zeev Gross, Harry B. Gray, Karn Sorasaenee, Daniel L. Farkas, "Large field of view scanning fluorescence lifetime imaging system for multimode optical imaging of small animals", Proc. SPIE 6859, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI, 68590G (18 March 2008); doi: 10.1117/12.769305; http://dx.doi.org/10.1117/12.769305
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:84667
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:05 Feb 2018 23:38
Last Modified:05 Feb 2018 23:38

Repository Staff Only: item control page