A Caltech Library Service

Residue-Specific Incorporation of Noncanonical Amino Acids for Protein Engineering

van Eldijk, Mark B. and van Hest, Jan C. M. (2018) Residue-Specific Incorporation of Noncanonical Amino Acids for Protein Engineering. In: Noncanonical Amino Acids. Methods in Molecular Biology. No.1728. Humana Press , New York, NY, pp. 137-145. ISBN 978-1-4939-7573-0.

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item:


The incorporation of noncanonical amino acids has given protein chemists access to an expanded repertoire of amino acids. This methodology has significantly broadened the scope of protein engineering allowing introduction of amino acids with non-native functionalities, such as bioorthogonal reactive handles (azides and alkynes) and hydrophobic fluorinated side chains. Here, we describe the efficient residue-specific replacement of methionine by azidonorleucine in an engineered green fluorescent protein using a bacterial expression system to introduce a single reactive site for the strain-promoted azide-alkyne cycloaddition.

Item Type:Book Section
Related URLs:
URLURL TypeDescription
Additional Information:© 2018 Springer Science+Business Media, LLC. This work was financially supported by Netherlands Organisation for Scientific Research (Rubicon grant 680-50-1407 and Gravitation program 024.001.035). The plasmids encoding the mutant MetRS and GFP were a kind gift from Prof. D. A. Tirrell (California Institute of Technology).
Funding AgencyGrant Number
Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO)680-50-1407
Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO)024.001.035
Subject Keywords:Noncanonical amino acids; Residue-specific incorporation; Aminoacyl-tRNA synthetase; Azidonorleucine; Medium shift; Bioconjugation; Strain-promoted azide-alkyne cycloaddition; Unnatural amino acids
Series Name:Methods in Molecular Biology
Issue or Number:1728
Record Number:CaltechAUTHORS:20180212-075402348
Persistent URL:
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:84776
Deposited By: Tony Diaz
Deposited On:13 Feb 2018 18:31
Last Modified:03 Oct 2019 19:22

Repository Staff Only: item control page