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Suppression of Cyclobutane and <6-4> Dipyrimidines Formation in Triple-Stranded H-DNA

Tang, Moon Shong and Htun, Han and Cheng, Yan and Dahlberg, James E. (1991) Suppression of Cyclobutane and <6-4> Dipyrimidines Formation in Triple-Stranded H-DNA. Biochemistry, 30 (28). pp. 7021-7026. ISSN 0006-2960. doi:10.1021/bi00242a030.

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We have determined the effect of H-DNA formation on the distributions of two ultraviolet (UV) light induced photoproducts-cyclobutane dipyrimidines and <6-4> dipyrimidines. A region of DNA containing the sequence (dT-dC)_(18)^*(dA-dG)_(18) was treated under conditions that specifically yield the triple-stranded H-y3 or H-y5 DNA structure and then irradiated with UV. The positions of cyclobutane dipyrimidines and <6-4> dipyrimidines were determined by T4 endonuclease V cleavage and by hot piperidine cleavage, respectively. Formation of H-DNA structures greatly decreased the photoproduct yield in the (dT-dC)_(18)^*(dA-dG)_(18) region but not elsewhere in the DNA. Suppression of photoproduct formation is greater in half of the repeat, reflecting whether the DNA is in the H-y3 or H-y5 conformation. Within the repeat, the suppression was less in the middle and toward the ends. Models for the suppression of photoproduct formation in H-DNA and the possible utility of our findings are discussed.

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Additional Information:© 1991 American Chemical Society. Published in print 16 July 1991. This research was supported by US. Public Health Service Grants ES 03124 and CA 42897 (MA T.) and GM 30220 (J.E.D.); Y. C. was supported by a grant of a gift from the Lucille P. Markey Charitable Trust, and H.H. was supported by an NIH postdoctoral fellowship (GM 14220). We thank Mr. B. Alderete and Dr. J. Pierce for their excellent technical assistance and Drs. M. Nazimeic, M. Patrick, and J. Pierce for their critical review of the manuscript.
Funding AgencyGrant Number
NIHES 03124
NIHCA 42897
NIHGM 30220
Lucille P. Markey Charitable TrustUNSPECIFIED
NIH Postdoctoral FellowshipGM 14220
Issue or Number:28
Record Number:CaltechAUTHORS:20180212-101922604
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Official Citation:Suppression of Cyclobutane and <6-4> Dipyrimidines Formation in Triple-Stranded H-DNA Moon Shong Tang, Han Htun, Yan Cheng, and James E. Dahlberg Biochemistry 1991 30 (28), 7021-7026 DOI: 10.1021/bi00242a030
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:84788
Deposited By: Ruth Sustaita
Deposited On:13 Feb 2018 21:32
Last Modified:15 Nov 2021 20:22

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