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Three-Dimensional Solution Structure of Saccharomyces cerevisiae Reduced Iso-l-cytochromec

Baistrocchi, Paolo and Banci, Lucia and Bertini, Ivano and Turano, Paola and Bren, Kara L. and Gray, Harry B. (1996) Three-Dimensional Solution Structure of Saccharomyces cerevisiae Reduced Iso-l-cytochromec. Biochemistry, 35 (43). pp. 13788-13796. ISSN 0006-2960. doi:10.1021/bi961110e. https://resolver.caltech.edu/CaltechAUTHORS:20180212-114157704

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Abstract

Two-dimensional ^1H NMR spectra of Saccharomyces cerevisiae reduced iso-1-cytochrome c have been used to confirm and slightly extend the assignment available in the literature. 1702 NOESY cross-peaks have been assigned, and their intensities have been measured. Through the program DIANA and related protocols (Güntert, 1992), a solution structure has been obtained by using 1442 meaningful NOEs and 13 hydrogen-bond constraints. The RMSD values with respect to the mean structure for the backbone and all heavy atoms for a family of 20 structures are 0.61 ± 0.09 and 0.98 ± 0.09 Å, the average target function value being as small as 0.57 Å^2. The larger number of slowly exchanging amide NHs observed in this system compared to that observed in the cyanide derivative of oxidized Ala 80 cytochrome c suggests that the oxidized form is much more flexible and that the backbone protons are more solvent accessible. Comparison of the present structure with the crystal structures of reduced yeast cytochrome c and of the complex between cytochrome c peroxidase and oxidized yeast cytochrome c reveals substantial similarity among the backbone conformations but differences in the residues located in the region of protein−protein interaction. Interestingly, in solution the peripheral residues involved in the interaction with cytochrome c peroxidase are on average closer to the position found in the crystal structure of the complex than to the solid state structure of the isolated reduced form.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/bi961110eDOIArticle
https://pubs.acs.org/doi/10.1021/bi961110ePublisherArticle
https://pubs.acs.org/doi/suppl/10.1021/bi961110ePublisherSupporting Information
ORCID:
AuthorORCID
Turano, Paola0000-0002-7683-8614
Gray, Harry B.0000-0002-7937-7876
Additional Information:© 1996 American Chemical Society. Received 8 May 1996. Published online 29 October 1996. This work was supported by CNR (Italy) and NSF (U.S.A.); it was performed with the instrumentation of the Florence Laboratory of Relaxometry and Magnetic Resonance on Paramagnetic Metalloproteins, Large Scale Facility of the European Community (Contract No. ERBCHGECT940060).
Funders:
Funding AgencyGrant Number
Consiglio Nazionale delle Ricerche (CNR)UNSPECIFIED
NSFUNSPECIFIED
European CommunityERBCHGECT940060
Issue or Number:43
DOI:10.1021/bi961110e
Record Number:CaltechAUTHORS:20180212-114157704
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180212-114157704
Official Citation:Three-Dimensional Solution Structure of Saccharomyces cerevisiae Reduced Iso-1-cytochrome c Paolo Baistrocchi, Lucia Banci, Ivano Bertini, Paola Turano, Kara L. Bren and Harry B. Gray Biochemistry 1996 35 (43), 13788-13796 DOI: 10.1021/bi961110e
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:84790
Collection:CaltechAUTHORS
Deposited By:INVALID USER
Deposited On:13 Feb 2018 21:25
Last Modified:15 Nov 2021 20:22

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