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Recombinantly Expressed Gas Vesicles as Nanoscale Contrast Agents for Ultrasound and Hyperpolarized MRI

Farhadi, Arash and Ho, Gabrielle and Kunth, Martin and Ling, Bill and Lakshmanan, Anupama and Lu, George and Bourdeau, Raymond W. and Schröder, Leif and Shapiro, Mikhail G. (2018) Recombinantly Expressed Gas Vesicles as Nanoscale Contrast Agents for Ultrasound and Hyperpolarized MRI. AIChE Journal, 64 (8). pp. 2927-2933. ISSN 0001-1541. PMCID PMC6289519. https://resolver.caltech.edu/CaltechAUTHORS:20180226-134658661

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Abstract

Ultrasound and hyperpolarized magnetic resonance imaging enable the visualization of biological processes in deep tissues. However, few molecular contrast agents are available to connect these modalities to specific aspects of biological function. We recently discovered that a unique class of gas‐filled protein nanostructures known as gas vesicles could serve as nanoscale molecular reporters for these modalities. However, the need to produce these nanostructures via expression in specialized cultures of cyanobacteria or haloarchaea limits their broader adoption by other laboratories and hinders genetic engineering of their properties. Here, we describe recombinant expression and purification of Bacillus megaterium gas vesicles using a common laboratory strain of Escherichia coli, and characterize the physical, acoustic, and magnetic resonance properties of these nanostructures. Recombinantly expressed gas vesicles produce ultrasound and hyperpolarized ^(129)Xe MRI contrast at subnanomolar concentrations, thus validating a simple platform for their production and engineering.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1002/aic.16138DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6289519PubMed CentralArticle
ORCID:
AuthorORCID
Farhadi, Arash0000-0001-9137-8559
Kunth, Martin0000-0002-9741-9881
Lakshmanan, Anupama0000-0002-6702-837X
Lu, George0000-0002-4689-9686
Bourdeau, Raymond W.0000-0003-2202-1980
Schröder, Leif0000-0003-4901-0325
Shapiro, Mikhail G.0000-0002-0291-4215
Additional Information:© 2018 American Institute of Chemical Engineers. Issue Online: 25 July 2018; Version of Record online: 08 March 2018; Accepted manuscript online: 23 February 2018; Manuscript revised: 07 February 2018; Manuscript received: 10 November 2017. This research was supported by the NIH (R01-EB018975 to MGS) and HFSP (RGP0050 to MGS and LS). A.L. is supported by the NSF graduate research fellowship (award number 1144469) and the NIH Biotechnology Leaders Program. A.F. is supported by the NSERC graduate fellowship. Related work in the Shapiro Lab is also supported by the Heritage Medical Research Institute, the Pew Charitable Trust, the Sontag Foundation, the Burroughs Wellcome Fund and the Packard Foundation.
Group:Heritage Medical Research Institute
Funders:
Funding AgencyGrant Number
NIHR01-EB018975
Human Frontier Science ProgramRGP0050
NSF Graduate Research FellowshipDGE-1144469
NIHUNSPECIFIED
Natural Sciences and Engineering Research Council of Canada (NSERC)UNSPECIFIED
Heritage Medical Research InstituteUNSPECIFIED
Pew Charitable TrustUNSPECIFIED
Sontag FoundationUNSPECIFIED
Burroughs Wellcome FundUNSPECIFIED
David and Lucile Packard FoundationUNSPECIFIED
Subject Keywords:acoustic nanostructures; gas vesicles; genetic engineering; magnetic resonance imaging; ultrasound
Issue or Number:8
PubMed Central ID:PMC6289519
Record Number:CaltechAUTHORS:20180226-134658661
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180226-134658661
Official Citation:Farhadi, A. , Ho, G. , Kunth, M. , Ling, B. , Lakshmanan, A. , Lu, G. J., Bourdeau, R. W., Schröder, L. and Shapiro, M. G. (2018), Recombinantly expressed gas vesicles as nanoscale contrast agents for ultrasound andhyperpolarized MRI. AIChE J., 64: 2927-2933. doi:10.1002/aic.16138
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:84956
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:26 Feb 2018 22:07
Last Modified:22 Nov 2019 20:34

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