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The combined absence of NF-kappa B1 and c-Rel reveals that overlapping roles for these transcription factors in the B cell lineage are restricted to the activation and function of mature cells

Pohl, Thomas and Gugasyan, Raffi and Grumont, Raelene J. and Strasser, Andreas and Metcalf, Donald and Tarlinton, David and Sha, William and Baltimore, David and Gerondakis, Steve (2002) The combined absence of NF-kappa B1 and c-Rel reveals that overlapping roles for these transcription factors in the B cell lineage are restricted to the activation and function of mature cells. Proceedings of the National Academy of Sciences of the United States of America, 99 (7). pp. 4514-4519. ISSN 0027-8424. https://resolver.caltech.edu/CaltechAUTHORS:POHpnas02

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Abstract

Transcription factors NF-KB1 and c-Rel, individually dispensable during embryogenesis, serve similar, yet distinct, roles in the function of mature hemopoietic cells. Redundancy among Rel/ NF-KB family members prompted an examination of the combined roles of c-Rel and NF-KB1 by using mice that lack both proteins. Embryonic development and the maturation of hemopoietic progenitors were unaffected in nfkb1(-/-)c-rel(-/-) mice. Peripheral T cell populations developed normally, but follicular, marginal zone, and CD5(+) peritoneal B cell populations all were reduced. In culture, a failure of mitogen-stimulated nfkb1(-/-)c-rel(-/-) B cells to proliferate was caused by a cell cycle defect in early G(1) that prevented growth. In vivo, defects in humoral immunity and splenic architecture seen in nfkbl(-/-) and c-rel(-/-) mice were exacerbated in the double mutant mice. These findings demonstrate that in the B lineage overlapping roles for NF-K81 and c-Rel appear to be restricted to regulating the activation and function of mature cells.


Item Type:Article
ORCID:
AuthorORCID
Baltimore, David0000-0001-8723-8190
Additional Information:Copyright © 2002 by the National Academy of Sciences. Communicated by Gustav J. Nossal, University of Melbourne, Melbourne, Australia, February 7, 2002 (received for review October 24, 2001). This work was supported by the National Health and Medical Research Council (Australia), the Anti-Cancer Council of Victoria, and the Leukemia and Lymphoma Society of America.
Subject Keywords:NF-KAPPA-B, LYMPHOCYTE-ACTIVATION, SUBUNIT COMPOSITION, IMMUNE-RESPONSES, EXHIBIT DEFECTS, B-1 CELLS, DIFFERENTIATION, EXPRESSION, PROTEINS, MICE
Issue or Number:7
Record Number:CaltechAUTHORS:POHpnas02
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:POHpnas02
Alternative URL:http://dx.doi.org/10.1073/pnas.072071599
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:851
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:26 Oct 2005
Last Modified:02 Oct 2019 22:37

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