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Cholesterol provides nonsacrificial protection of membrane lipids from chemical damage at air–water interface

Zhang, Xinxing and Barraza, Kevin M. and Beauchamp, J. L. (2018) Cholesterol provides nonsacrificial protection of membrane lipids from chemical damage at air–water interface. Proceedings of the National Academy of Sciences of the United States of America, 115 (13). pp. 3255-3260. ISSN 0027-8424. PMCID PMC5879709. https://resolver.caltech.edu/CaltechAUTHORS:20180305-160808428

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Abstract

The role of cholesterol in bilayer and monolayer lipid membranes has been of great interest. On the biophysical front, cholesterol significantly increases the order of the lipid packing, lowers the membrane permeability, and maintains membrane fluidity by forming liquid-ordered–phase lipid rafts. However, direct observation of any influence on membrane chemistry related to these cholesterol-induced physical properties has been absent. Here we report that the addition of 30 mol % cholesterol to 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) or 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (POPG) monolayers at the air–water interface greatly reduces the oxidation and ester linkage cleavage chemistries initiated by potent chemicals such as OH radicals and HCl vapor, respectively. These results shed light on the indispensable chemoprotective function of cholesterol in lipid membranes. Another significant finding is that OH oxidation of unsaturated lipids generates Criegee intermediate, which is an important radical involved in many atmospheric processes.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1073/pnas.1722323115DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5879709//PubMed CentralArticle
ORCID:
AuthorORCID
Barraza, Kevin M.0000-0003-1849-5219
Beauchamp, J. L.0000-0001-8839-4822
Additional Information:© 2018 the Author(s). Published under the PNAS license. Contributed by J. L. Beauchamp, February 6, 2018 (sent for review December 21, 2017; reviewed by Barbara J. Finlayson-Pitts and Richard N. Zare) published ahead of print March 5, 2018, https://doi.org/10.1073/pnas.1722323115 This work was supported by the Beckman Institute at Caltech and by NSF Grant CHE-1508825. Author contributions: X.Z. and J.L.B. designed research; X.Z. and K.M.B. performed research; X.Z. performed calculations; X.Z. and J.L.B. analyzed data; and X.Z. and J.L.B. wrote the paper. The authors declare no conflict of interest.
Funders:
Funding AgencyGrant Number
Caltech Beckman InstituteUNSPECIFIED
NSFCHE-1508825
Subject Keywords:cholesterol; phospholipid; OH radical; chemoprotection; air–water interface
Issue or Number:13
PubMed Central ID:PMC5879709
Record Number:CaltechAUTHORS:20180305-160808428
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180305-160808428
Official Citation:Cholesterol provides nonsacrificial protection of membrane lipids from chemical damage at air–water interface Xinxing Zhang, Kevin M. Barraza, J. L. Beauchamp Proceedings of the National Academy of Sciences Mar 2018, 115 (13) 3255-3260; DOI: 10.1073/pnas.1722323115
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:85113
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:06 Mar 2018 16:31
Last Modified:17 Dec 2019 18:25

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