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Organization of kappa light chain genes in germ-line and somatic tissue

Joho, Rolf and Weissman, Irving L. and Early, Philip and Cole, Jim and Hood, Leroy (1980) Organization of kappa light chain genes in germ-line and somatic tissue. Proceedings of the National Academy of Sciences of the United States of America, 77 (2). pp. 1106-1110. ISSN 0027-8424.

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We studied the organization of the light chain genes in germ-line (sperm) and somatic (embryo) tissues. We constructed a plasmid containing a DNA insert coding for the kappa chain MOPC 167 and used the Southern blotting technique to determine the organization of variable and constant region genes. In the haploid genome of the mouse there is only one constant region gene detectable and it has the same organization in sperm and embryo DNAs. There are several variable region genes in sperm and embryo that are related to the Vk167 gene. The organization of the V genes in sperm and embryo DNAs is identical. These results show that there is no rearrangement of variable region genes (or "minigenes") during early embryogenesis.

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Hood, Leroy0000-0001-7158-3678
Additional Information:© 1980 by the National Academy of Sciences. Communicated by H. N. Eisen, October 5, 1979. This work was supported by American Cancer Society Grant IM56 (to I.L.W.) and National Science Foundation Grant PCM 76-81542 (to L.H.). I.L.W. is a Faculty Research Awardee of the American Cancer Society. R.J. is Postdoctoral Fellow of the Swiss National Foundation. P.E. is supported by National Institutes of Health Training Grant GM 07616. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:antibody diversity; germ-line genes; restriction map
Issue or Number:2
Record Number:CaltechAUTHORS:JOHpnas80a
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:8523
Deposited By: Tony Diaz
Deposited On:17 Aug 2007
Last Modified:02 Oct 2019 23:51

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