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Deconvoluting the effects of surface chemistry and nanoscale topography: Pseudomonas aeruginosa biofilm nucleation on Si-based substrates

Zhang, Jing and Huang, Jingling and Say, Carmen and Dorit, Robert L. and Queeney, K. T. (2018) Deconvoluting the effects of surface chemistry and nanoscale topography: Pseudomonas aeruginosa biofilm nucleation on Si-based substrates. Journal of Colloid and Interface Science, 519 . pp. 203-213. ISSN 0021-9797 . http://resolver.caltech.edu/CaltechAUTHORS:20180312-082018611

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Abstract

Hypothesis: The nucleation of biofilms is known to be affected by both the chemistry and topography of the underlying substrate, particularly when topography includes nanoscale (<100 nm) features. However, determining the role of topography vs. chemistry is complicated by concomitant variation in both as a result of typical surface modification techniques. Analyzing the behavior of biofilm-forming bacteria exposed to surfaces with systematic, independent variation of both topography and surface chemistry should allow differentiation of the two effects. Experiments: Silicon surfaces with reproducible nanotopography were created by anisotropic etching in deoxygenated water. Surface chemistry was varied independently to create hydrophilic (OH-terminated) and hydrophobic (alkyl-terminated) surfaces. The attachment and proliferation of Psuedomonas aeruginosa to these surfaces was characterized over a period of 12 h using fluorescence and confocal microscopy. Findings: The number of attached bacteria as well as the structural characteristics of the nucleating biofilm were influenced by both surface nanotopography and surface chemistry. In general terms, the presence of both nanoscale features and hydrophobic surface chemistry enhance bacterial attachment and colonization. However, the structural details of the resulting biofilms suggest that surface chemistry and topography interact differently on each of the four surface types we studied.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.jcis.2018.02.068DOIArticle
https://www.sciencedirect.com/science/article/pii/S0021979718302248PublisherArticle
Additional Information:© 2018 Elsevier Inc. Received 17 November 2017, Revised 21 February 2018, Accepted 22 February 2018, Available online 24 February 2018.
Subject Keywords:Biofilm nucleation; Nanoscale; Silicon; Surface chemistry; Surface topography; Pseudomonas aeruginosa
Record Number:CaltechAUTHORS:20180312-082018611
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20180312-082018611
Official Citation:Jing Zhang, Jingling Huang, Carmen Say, Robert L. Dorit, K.T. Queeney, Deconvoluting the effects of surface chemistry and nanoscale topography: Pseudomonas aeruginosa biofilm nucleation on Si-based substrates, Journal of Colloid and Interface Science, Volume 519, 2018, Pages 203-213, ISSN 0021-9797, https://doi.org/10.1016/j.jcis.2018.02.068. (http://www.sciencedirect.com/science/article/pii/S0021979718302248)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:85238
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:12 Mar 2018 17:17
Last Modified:12 Mar 2018 17:17

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